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Iranian Journal of Public Health، جلد ۴۹، شماره ۳، صفحات ۵۵۷-۵۶۲
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عنوان انگلیسی Investigation of JC Polyomavirus (JCV) Genome in Colorectal Cancer Patients from Iran
چکیده انگلیسی مقاله Background: JC polyomavirus (JCV) is an epitheliotropic and neurotropic virus that identified in relationship with some devastating complications such as progressive multifocal leukoencephalopathy (PML) and linked to colorectal cancer. The aim of current study was to identify the prevalence of JCV in colorectal cancer for the first time in Iran. Methods: This retrospective case-control study was conducted by the hospitals affiliated to Iran University of Medical Sciences, Tehran, Iran from 2011 to 2016. Formalin-fixed paraffin-embedded (FFPE) blocks were used for DNA extraction by QIAamp® DNA FFPE Tissue Kit. The SYBER Green Real-time PCR assay performed by specific primers for JCV T-Large Ag. Melting curve analysis used for evaluation of amplification specificity. Positive control cloned in pTZ57R/T plasmid by Generay Biotechnology system. Results: Of 157 specimens 66 were colorectal cancer by the mean age (y) ± std. deviation 59.35±14.48 and 91 healthy control by the mean age (y) ± std. deviation 57.21±14.66. All 157 specimens tested for JCV T-Large Ag gene by Real-time PCR method and we found that there was not any positive result although the melting analysis showed specificity of positive control amplification. Conclusion: Low prevalence of JCV infection in Iranian CRC population confirmed by the current study results; there was not any JCV positive result in CRC and healthy control groups. Further studies by broader and different populations are recommended.  
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نویسندگان مقاله | Mohammad Hadi KARBALAIE NIYA
Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran


| Mohsen KESHAVARZ
The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran


| Fahimeh SAFARNEZHAD TAMESHKEL
1. Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran 2. Student Research Committee, Iran University of Medical Sciences, Tehran, Iran


| Mahsa TAHERIZADEH
Department of Virology, Pasteur Institute of Iran, Tehran, Iran


| Maryam ESGHAEI
Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran


| Mahshid PANAHI
Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran


| Hossein KEYVANI
1. Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran 2. Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
نشانی اینترنتی http://ijph.tums.ac.ir/index.php/ijph/article/view/19911
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/86/article-86-2305642.pdf
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