| چکیده انگلیسی مقاله |
Background: Obesity is a complex disorder influenced by various genetic and environmental factors. It has been shown that gut microbiota, which colonizes gastrointestinal tract, has a substantial role as an environmental factor in the pathophysiology of obesity. Since the composition of gut microbiota alters with regard to different criteria, such as ethnicity, geographical location, diet, lifestyle, age, and gender, we aimed to determine F/B ratio and the abundance of important gut microbiota members, A. muciniphila, F. prausnitzii, Roseburia, Bifidobacterium, and Prevotella in Iranian obese and normal weight individuals, for the first time. Methods: In this study, 50 normal and 50 obese subjects were recruited and classified based on their BMI into normal weight and obese groups. Stool samples were collected. Following DNA extraction from the samples, qPCR was conducted based on 16s rDNA universal primers. Finally, the correlation between the bacterial abundance and obesity was analyzed by statistical analyses. Results: We observed a significant increase of F/B ratio in the obese group, compared to the normal weight group (p = 0.002). Although A. muciniphila (p = 0.039) and Bifidobacterium (p = 0.049) abundance significantly decreased, the abundance of F. prausnitzii (p = 0.046) significantly elevated with BMI increase in the studied groups. Conclusion: Owing to the importance of the gut microbiota composition in obesity development, determination and targeted restoration of gut microbiota pattern could be valuable in the control and treatment of obesity in certain populations. |
| نویسندگان مقاله |
| Fateme Ettehad Marvasti
Microbiology Research Centre, Pasteur Institute of Iran, Tehran, Iran
| Arfa Moshiri
Research Centre for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| Mina Sadat Taghavi
The Islamic Azad University, Science and Research Branch, Tehran, Iran
| Soheil Riazi
The Islamic Azad University, Science and Research Branch, Tehran, Iran
| Majid Taati
The Islamic Azad University, Science and Research Branch, Tehran, Iran
| Seyedeh Fatemeh Sadati
The Islamic Azad University, Science and Research Branch, Tehran, Iran
| Azadeh Ghaheri
Departments of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
| Morteza Masoomi
Microbiology Research Centre, Pasteur Institute of Iran, Tehran, Iran
| Farzam Vaziri
Microbiology Research Centre, Pasteur Institute of Iran, Tehran, Iran
| Abolfazl Fateh
Microbiology Research Centre, Pasteur Institute of Iran, Tehran, Iran
| Pejman Rohani
Department of Pediatric Gastroentrology and Hepatology, Mofid Children's Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| Samira Tarashi
Microbiology Research Centre, Pasteur Institute of Iran, Tehran, Iran
| Andrea Masotti
Research Laboratories, Bambino Gesù Children's Hospital-IRCCS, Rome, Italy
| Sara Ahmadi Badi
Microbiology Research Centre, Pasteur Institute of Iran, Tehran, Iran
| Seyed Davar Siadat
Microbiology Research Centre, Pasteur Institute of Iran, Tehran, Iran
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