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Iranian Biomedical Journal، جلد ۲۴، شماره ۳، صفحات ۱۷۳-۱۸۲
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عنوان انگلیسی In silico Evaluation of PLAC1-fliC As a Chimeric Vaccine against Breast Cancer
چکیده انگلیسی مقاله Background: Breast cancer is one of the most prevalent cancers among women. Common cancer treatment methods are not effective enough, and there is a need for a more efficient treatment procedure. Cancer vaccine is a novel immunotherapy method that stimulates humoral and/or cellular immunity against cancer. Placenta-specific protein 1 (PLAC1) is a cancer/testis antigen, prevalent in breast cancer and rarely found in normal tissues. FliC as a bacterial adjuvant when fused to PLAC1 can elicit humoral and cellular responses. Therefore, PLAC1-fliC is a chimeric protein, which can be considered a suitable candidate against breast cancer. Methods: ProtParam was used to evaluate physicochemical properties of PLAC1-fliC. Second structures were determined using the GOR V server. PLAC1-fliC three-dimensional structure was modeled by Phyre2, and it was refined using GalaxyWEB. The refined model was submitted to RAMPAGE, PROCHECK, and ProSA-web for validation. Antigenicity and allergenicity of the construct were predicted by ANTIGENpro, VaxiJen, AllergenFP, and SDAP databases. Then MHC‑I- and MHC‑II-binding epitopes of PLAC1-fliC were forecasted by NetMHC 4.0 and NetMHCII 2.3 Servers. Finally, Ellipro and CTLpred were employed to predict B cell and cytotoxic T lymphocytes epitopes. Results: The construct was evaluated as a stable fusion protein, which could be antigenic and could stimulate B and T cells against breast cancer. Conclusion: PLAC1-fliC, as a cancer vaccine candidate, might be suitable and specific for breast cancer, which could evoke humoral and cellular immunity against this type of tumor.
کلیدواژه‌های انگلیسی مقاله Breast cancer, PLAC1, Cancer vaccines, Bioinformatics
نویسندگان مقاله | Mortaza Taheri-Anganeh
Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran


| Ahmad Amiri
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran


| Ahmad Movahedpour
Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran


| Seyyed Hossein Khatami
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran


| Younes Ghasemi
Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran


| Amir Savardashtaki
Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran


| Zohreh Mostafavi-Pour
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-796&slc_lang=other&sid=1
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/125/article-125-2326205.pdf
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