این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Microbiology، جلد ۱۲، شماره ۲، صفحات ۷۷-۸۸
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Immuno-proteomics analysis between OMV of vaccine and dominant wild type strains of Bordetella pertussis in Iran
چکیده انگلیسی مقاله Background and Objectives: Despite widespread vaccination programs against pertussis, there has been a worldwide resurgence of the disease in recent years. We aimed to investigate protein composition of outer membrane vesicles (OMV) of Bordetella pertussis (Bp) and to evaluate the immunogenicity of OMV antigens both in the vaccine and the dominant wild type strains in Iran. Materials and Methods: The OMV were purified from both vaccine and wild type strains. The immunoreactivity of the OMVs was investigated by exposing sera taken from the patients and the vaccinated infants. The protein profiles of OMVs were compared using two-dimensional electrophoresis. The LC-MS/MS was used to analyse and identify differentially expressed protein spots. Results: The two type strains showed differences in their 2D gel protein profile. Further analysis of selected proteins from the dominant Iranian strains using LC-MS/MS demonstrated that the identified proteins fell into different functional categories including (i) metabolism, (ii) membrane transport and secretion system, (iii) biosynthesis and degradation, (iv) adaption, adhesion, pathogenicity, conserved hypothetical and protection responses. Moreover, a number of immunogenic proteins were identified including Bp 2434 (serine protease) and Bp 1616 (putative DNA binding protein) from the vaccine and the wild type strains, respectively which could be considered as potential antigens for an OMV vaccine. Conclusion: OMV Bp could be considered as an alternative vaccine against pertussis, containing the bacterium’s protein antigens that can confer equal efficacy compared to a whole bacterial cell vaccine with advantages such as less side effects and lower costs than acellular pertussis vaccines.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Ali Badamchi
Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran


| Fariborz Bahrami
Department of Immunology, Pasteur Institute of Iran, Tehran, Iran


| Alireza Hadizadeh Tasbiti
Department of Tuberculosis and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran


| Shamsi Yari
Department of Tuberculosis and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran


| Morvarid Shafiei
Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran


| Fereshteh Shahcheraghi
Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran


| Seyed Davar Siadat
Department of Tuberculosis and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran
نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/2234
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/91/article-91-2358777.pdf
کد مقاله (doi)
زبان مقاله منتشر شده
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات