این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Microbiology، جلد ۱۲، شماره ۲، صفحات ۱۳۸-۱۴۷
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Aerobic dichlorvos degradation by Pseudomonas stutzeri smk: complete pathway and implications for toxicity in Mus musculus
چکیده انگلیسی مقاله Background and Objectives: Excess use of pesticides in agricultural field not only compromised soil fertility but also posed serious threat to water bodies and life in the surrounding environment. The leftover pesticide residue needs to be remediated effectively. Compared to physical, chemical and enzymatic remediation options the microbial remediation is more practical and sustainable. Materials and Methods: The Pseudomonas stutzeri smk strain was found to use dichlorvos as the solitary carbon source. Minimal medium supplemented with dichlorvos was used to test ability of bacterium to degrade pesticide aerobically. The metabolites produced by the bacterium were studied with UV-Vis spectrophotometry, HPLC, FTIR and GC-MS techniques. The toxicity studies of neat dichlorvos and P. stutzeri smk degraded metabolites were studied by subcutaneous injection in Mus musculus. Results: The P. stutzeri smk strain was found to degrade as high as 80% of dichlorvos on 7th day of incubation, at 30 °C temperature and at pH 7. In five steps complete aerobic degradation of 2,2dicholorvinyl dimethyl phosphate (dichlorvos) resulted in production of free methyl and phosphate. The degradation intermediates produced are 2-Chlorovinyl dimethyl phosphate, vinyl dimethyl phosphate, dimethyl phosphate, methylphosphate and finally free phosphate. The histopathological analysis of liver, spleen and thymus of M. musculus were performed to study toxicity of dichlorvos and degraded metabolites. Conclusion: P. stutzeri smk could result highest aerobic degradation of dichlorvos to produce free methyl and phosphate. Degradation metabolites could reverse largely toxic effects of dichlorvos when studied in M. musculus.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Satish Parte
Department of Zoology, M. J. S. College, Shrigonda, Ahmednagar, India


| Ashok Mohekar
Department of Zoology, Maharshi Dnyandeo Mohekar Mahavidyalaya, Kallamb, Osmanabad, India


| Arun Kharat
Laboratory of Applied Microbiology, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India
نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/2407
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/91/article-91-2358784.pdf
کد مقاله (doi)
زبان مقاله منتشر شده
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات