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Physiology and Pharmacology، جلد ۲۵، شماره ۳، صفحات ۲۷۹-۲۸۷
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عنوان انگلیسی Nicotine effects on enzymatic antioxidant defenses in human breast and ovarian cell lines
چکیده انگلیسی مقاله Introduction: Nowadays, the prevalence of nicotine abuse among women has increased dramatically. In the current study, we aimed to investigate the effect of nicotine exposure on breast MCF-7 and ovarian OVCAR-3 cell lines for assessing the toxicity of nicotine in the cells of these organs. Methods: The MCF-7 and OVCAR-3 cells were treated with increasing nicotine concentrations ranging from 0 (control), 10-11, 10-8 and 10-6 M for 24h. Effect of nicotine treatments on major antioxidant enzymes catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), cellular levels of glutathione (GSH) and malondialdehyde (MDA) were monitored. Results: We showed that the CAT activity in MCF-7 cells increased only at 10-6 M dose of nicotine. The GPx and GR activity was decreased at 10-8 and 10-6 M of nicotine in MCF-7 cells, but in OVCAR-3 cells, this decrease was significant only at 10-6 M dose of nicotine. Reduced GSH decrease was statistically significant only at 10-8 and 10-6 M of nicotine in MCF-7 cells; otherwise, in OVCAR-3 cells, this decline was significant only at 10-6 M of nicotine. Nicotine at 10-8 and 10-6 M concentration caused a significant increase in MDA levels in MCF-7 cells. Conclusion: This study showed that breast MCF-7 cells are more vulnerable than ovarian OVCAR-3 cells against nicotine-induced oxidative toxicity.
کلیدواژه‌های انگلیسی مقاله Nicotine, Antioxidant enzymes, Oxidative stress, MCF-7 cells, OVCAR-3 cells.
نویسندگان مقاله | Amir Yarahmadi
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran


| Neda Mohammadi
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran


| Fatemeh Zal
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
نشانی اینترنتی http://ppj.phypha.ir/browse.php?a_code=A-10-1357-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Cell, Stem Cell and Cancer
نوع مقاله منتشر شده Experimental research article
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