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International Journal of Radiation Research، جلد ۲۰، شماره ۱، صفحات ۱۵-۲۰
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عنوان انگلیسی LINC01204 Negatively Regulates the Effect of MiR-214 on Lung Cancer Cell Apoptosis, Migration, Invasion and Radio-sensitivity
چکیده انگلیسی مقاله Background: To investigate whether LncRNA LINC01204 affects lung cancer cell apoptosis, migration, invasion and radiosensitivity by regulating miR-214. Materials and Methods: Normal lung cells HBE and lung cancer cells A549 were cultured in-vitro, and pcDNA3.1, pcDNA3.1-LINC01204, pcDNA3.1-LINC01204 and miR-NC, pcDNA3.1-LINC01204 and miR-214 mimics were transfected into A549 cells, respectively. qRT-PCR, flow cytometry, scratch test, and Transwell chamber test, clone formation test, dual luciferase, and Western blot methods were used in this study. Results: Compared with HBE cells, A549 cells had significantly reduced LINC01204 expression level (P< 0.05), and significantly increased miR-214 expression level (P< 0.05); transfection of pcDNA3.1-LINC01204 could significantly increase apoptosis rate and Cleaved-caspase3, E-cadherin protein levels (P< 0.05), reduce migration healing rate, N-cadherin protein levels and cell survival fraction (P< 0.05) with sensitization enhancement ratio SER at 1.950, reduce the number of invasive cells (P< 0.05). Dual luciferase report experiments confirmed that LINC01204 can bind to miR-214 in a targeted way; co-transfection of pcDNA3.1-LINC01204 and miR-214 mimics can significantly reduce the apoptosis rate and Cleaved-caspase3 and E-cadherin protein levels (P< 0.05), improve the migration healing rate, N-cadherin protein level and cell survival fraction (P< 0.05) with sensitization enhancement ratio SER at 0.728, increase the number of invasive cells (P< 0.05). Conclusion: LINC01204 overexpression can negatively regulate miR-214 expression to promote lung cancer cell apoptosis and inhibit migration and invasion, thereby enhancing radiosensitivity.  
کلیدواژه‌های انگلیسی مقاله LncRNA LINC01204, miR-214, lung cancer, apoptosis, migration, invasion, radiosensitivity.
نویسندگان مقاله | X.N. Yu
Department of Oncology, Weihai Central Hospital, Weihai, 264400, China


| M. Wang
Department of Oncology, Weihai Central Hospital, Weihai, 264400, China
نشانی اینترنتی http://ijrr.com/browse.php?a_code=A-10-2188-55&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Radiobiology
نوع مقاله منتشر شده تحقیق بدیع
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