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International Journal of Radiation Research، جلد ۱۹، شماره ۳، صفحات ۶۴۵-۶۵۱
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عنوان انگلیسی Effects of radiation dose on the stemness-related genes expression in colorectal cancer cell line
چکیده انگلیسی مقاله Background: Accumulating reports suggest that radiation may change gene expression in cancer cells and promote cell migration and invasion, as well as inducing cancer stem cell (CSC). However, the correlation between these processes and radiation dose has not been shown yet. Therefore, the present study aimed to evaluate the effect of low, medium, and high doses of X-ray on expressing three genes involved in CSC induction in colon cancer cell line (HT-29). Materials and Methods: The cells cultured in flasks were irradiated with X- rays in different doses including 0.1, 2.5, 5, and 10 Gy. Then, the expression of Oct4, CD44, and ALDH1 genes was measured using real-time PCR. PCR efficiency was evaluated for each gene using Linreg PCR software, and relative changes for mRNA were calculated based on the ∆∆Ct method. Results: CD44 gene expression increased equally at all doses. Oct4 and ALDH1 gene expression were not affected by 10 Gy, but low and moderate doses increased them equally. Conclusion: The effects of low and moderate doses on increasing the expression of stem-related genes are equal. In addition, the effect of the high dose on increasing CD44 gene expression was equal to the low and moderate doses.
کلیدواژه‌های انگلیسی مقاله Radiation, colorectal cancer, gene expression, real-time PCR.
نویسندگان مقاله | Sh. Soleymanifard
Medical Physics Research Center, Mashhad University of Medical Science, Mashhad, Iran


| M. Rostamyari
Department of Medical Physics and Engineering, School of Medicine, Shiraz University of Medical Science, Shiraz, Iran


| N. Jaberi
Department of Biochemistry, School of Medicine, Mashhad University of Medical Science, Mashhad, Iran


| F.B. Rassouli
Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran


| S.I. Hashemy
Department of Biochemistry, School of Medicine, Mashhad University of Medical Science, Mashhad, Iran


| A.R. Mehdizadeh
Department of Medical Physics and Engineering, School of Medicine, Shiraz University of Medical Science, Shiraz, Iran
نشانی اینترنتی http://ijrr.com/browse.php?a_code=A-10-2188-39&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Radiobiology
نوع مقاله منتشر شده تحقیق بدیع
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