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Acta Medica Iranica، جلد ۵۸، شماره ۶، صفحات ۲۶۰-۲۶۵
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عنوان انگلیسی Inhibition of Colorectal Cancer Cell Line CaCo-2 by Essential Oil of Eucalyptus camaldulensis Through Induction of Apoptosis
چکیده انگلیسی مقاله Treatment of colorectal cancer is one of the important challenges due to the increase of resistance to chemotherapeutic drugs. Isolated natural compounds from medicinal plants and other sources often are used as novel drugs for treatment of various human cancer. The aim of this study was to investigate the antioxidant and anticancer activity of Eucalyptus camaldulensis essential oil on colorectal cancer cell line Caco-2. The antioxidant activity of extracted E. camaldulensis essential oil (1000, 800, 400, 200, 100, 50, 25, 12.5, 6, and 3 μg/mL) was evaluated by free radicals inactivation method. Moreover, MTT assay was used to examine the cytotoxic effects of E. camaldulensis essential oil on the Caco-2 cell line. The mRNA expression of BAX and BCL-2 genes was studied using quantitative Real-Time PCR method, in treated cancer cells compared to untreated cells. We indicated a significant, impressive antioxidant activity in 1000 μg/mL of E. camaldulensis essential oil, in a concentration-dependent manner. In addition, we found that this product exerted a cytotoxic effect on cancer cells when 100 μg/mL concentration was considered as half-maximal inhibitory concentration (IC50). Also, the expression of BAX and BCL-2 genes were significantly upregulated and downregulated, respectively, in the treated Caco-2 cells with E. camaldulensis essential oil. In conclusion, our study showed significant antioxidant and anticancer activity in E. camaldulensis essential oil in a concentration and time-dependent manner, which may be due to the reduction of free radicals and induction of apoptosis process in colorectal cancer cells.
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نویسندگان مقاله | Elham Taheri
Department of Pharmaceutical Biotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.


| Saba Ghorbani
Department of Genetics, Tabriz Branch, Islamic Azad University, Tabriz, Iran.


| Maryam Safi
Department of Genetics, Tabriz Branch, Islamic Azad University, Tabriz, Iran.


| Nasrin Seyyed Sani
Department of Genetics, Tabriz Branch, Islamic Azad University, Tabriz, Iran.


| Fatemeh Firouzi Amoodizaj
Department of Genetics, Tabriz Branch, Islamic Azad University, Tabriz, Iran.


| Masoud Heidari
Department of Neurology, Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran.


| Reza Chavoshi
Department of Genetics, Tabriz Branch, Islamic Azad University, Tabriz, Iran


| Saba Hajazimian
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.


| Alireza Isazadeh
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran


| Mansour Heidari
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
نشانی اینترنتی https://acta.tums.ac.ir/index.php/acta/article/view/8263
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