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Iranian Journal of Public Health، جلد ۵۰، شماره ۱۲، صفحات ۲۵۷۶-۲۵۸۳
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عنوان انگلیسی The Effect of SLC2A3 Expression on Cisplatin Resistance of Colorectal Cancer Cells
چکیده انگلیسی مقاله Background: To study the molecular mechanism of cisplatin chemotherapy resistance in colorectal cancer cells and to explore the effect of miRNA in regulating the expression of glucose transporter 3 (SLC2A3) and the proliferation and migration of colon cancer cells. Methods: All samples were obtained from the People’s Hospital of Wuhai, Wuhai, China between June 2019 and June 2020. Real-time quantitative PCR (qRT-PCR) was carried out to check the expression of miR-103a in these cell lines. Western blotting and Luciferase reporter gene detection confirmed the regulation of the miR-103a/SLC2A3 axis. Western blotting detected the activation of SLC2A3, caspased-9 and -3. Results: The expression of SLC2A3 protein in colon cancer cell lines was significantly higher than that of normal colon cancer cells, while the expression of SLC2A3 miRNA showed no significant difference (P< 0.05). Then, through clone formation analysis, SLC2A3 was closely related to the proliferation of human colon cancer cells. Functional recovery experiments showed that increasing the expression of miR-103a could reverse the abnormal proliferation caused by overexpression of SLC2A3. Conclusion: Overall, miR-103a can inhibit the proliferation of human colon cancer cells by targeting SLC2A3, and this result will provide a potential target for the treatment of colon cancer.
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نویسندگان مقاله | Yan Li
Digestive Internal, The People&#x2019;s Hospital of Wuhai, Wuhai, P.R. China


| Hailong Lei
Internal Medicine-Oncology, The People&#x2019;s Hospital of Wuhai, Wuhai, P.R. China


| Ming Zhang
General Surgery, The People&#x2019;s Hospital of Wuhai, Wuhai, P.R. China


| Guangming Wu
General Surgery, The People&#x2019;s Hospital of Wuhai, Wuhai, P.R. China


| Caiyun Guo
Digestive Internal, The People&#x2019;s Hospital of Wuhai, Wuhai, P.R. China


| Zijing Yang
Digestive Internal, The People&#x2019;s Hospital of Wuhai, Wuhai, P.R. China


| Jingting Zhang
Clinical Laboratory, The People&apos;s Hospital of Wuhai, Wuhai, P.R. China


| Yuan Wang
Clinical Laboratory, The People&apos;s Hospital of Wuhai, Wuhai, P.R. China


| Jianbin Zhu
Digestive Internal, The People&#x2019;s Hospital of Wuhai, Wuhai, P.R. China


| Yongzhe Du
Digestive Internal, The People&#x2019;s Hospital of Wuhai, Wuhai, P.R. China
نشانی اینترنتی https://ijph.tums.ac.ir/index.php/ijph/article/view/24829
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