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Iranian Journal of Basic Medical Sciences، جلد ۲۴، شماره ۱۲، صفحات ۱۷۳۴-۱۷۴۲
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عنوان انگلیسی Curcumin protects human umbilical vein endothelial cells against high oxidized low density lipoprotein-induced lipotoxicity and modulates autophagy
چکیده انگلیسی مقاله Objective(s): Endothelial dysfunction is a precursor of cardiovascular disease, and protecting endothelial cells from damage is a treatment strategy for atherosclerosis (AS). Curcumin, a natural polyphenolic compound, has been shown to protect endothelial cells from dysfunction. In the present study, we investigated whether curcumin could ameliorate high oxidized low-density lipoprotein (ox‐LDL)-induced endothelial lipotoxicity by inducing autophagy in human umbilical vein endothelial cells (HUVECs).Materials and Methods: HUVECs were treated with 50 μM high ox‐LDL alone or in combination with 5 μM curcumin for 24 hr. Cell viability and function were assessed by the cell counting kit-8 (CCK-8) assay, tube formation assay and cell migration experiments. Oil red O staining was used to detect lipid droplet accumulation in HUVECs. The change in reactive oxygen species (ROS) levels in HUVECs was measured with the probe DCFH-DA. Quantitative real-time PCR (qPCR) and Western blotting were used to evaluate the mRNA and protein levels of several inflammatory and autophagy-related factors.Results: Cell viability was restored, tube formation and migration ability were increased, and lipid accumulation, oxidative stress and inflammatory responses were decreased in the curcumin-treated group compared with the high ox‐LDL group. Furthermore, high ox‐LDL inhibited HUVEC autophagy, and this effect was reversed by curcumin. Moreover, curcumin regulated the expression of several key proteins involved in the AMPK/mTOR/p70S6K signaling pathway.Conclusion: Our findings suggest that curcumin is able to reduce endothelial lipotoxicity and modulate autophagy and that the AMPK/mTOR/p70S6K pathway might play a key role in these effects.
کلیدواژه‌های انگلیسی مقاله Atherosclerosis, Autophagy, Curcumin, Endothelial cells, Lipid metabolism disorders
نویسندگان مقاله | Lifeng Zhao
Department of Immunology and Microbiology, School of Basic Medical Sciences, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, P.R. China


| Ruixi Luo
Department of Immunology and Microbiology, School of Basic Medical Sciences, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, P.R. China


| Honghong Yu
Department of Experimental Center, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, P.R. China


| Shuaishuai Li
Department of Immunology and Microbiology, School of Basic Medical Sciences, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, P.R. China


| Wenjia Wang
Department of Immunology and Microbiology, School of Basic Medical Sciences, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, P.R. China


| Wenjia Wang
Department of Immunology and Microbiology, School of Basic Medical Sciences, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, P.R. China


| Kun Cai
Department of Immunology and Microbiology, School of Basic Medical Sciences, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, P.R. China


| Tao Xu
Department of Cardiovascular Medicine, Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 550025, P.R. China


| Rui Chen
Department of Morphology Laboratory, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, P.R. China


| Weiyi Tian
Department of Immunology and Microbiology, School of Basic Medical Sciences, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, P.R. China
نشانی اینترنتی https://ijbms.mums.ac.ir/article_19144.html
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