این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Basic Medical Sciences، جلد ۲۴، شماره ۱۲، صفحات ۱۷۵۳-۱۷۶۲
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Combined effect of hydrogen sulfide and mesenchymal stem cells on mitigating liver fibrosis induced by bile duct ligation: Role of anti-inflammatory, anti-oxidant, anti-apoptotic, and anti-fibrotic biomarkers
چکیده انگلیسی مقاله Objective(s): Liver fibrosis eventually develops into cirrhosis and hepatic failure, which can only be treated with liver transplantation. We aimed to assess the potential role of hydrogen sulfide (H2S) alone and combined with bone marrow-derived mesenchymal stem cells (BM-MSCs) on hepatic fibrosis induced by bile-duct ligation (BDL) and to compare their effects to silymarin.Materials and Methods: Alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TB), and alkaline phosphatase (ALP) were investigated in serum. Gene expression levels of CBS (cystathionine β-synthase), CSE (cystathionine γ-lyase), and alpha-smooth muscle actin (α- SMA) were measured in liver tissues using RT-PCR. Hepatic protein kinase (Akt) was assessed by Western blot assay. Liver oxidative stress markers, malondialdehyde (MDA), and reduced glutathione (GSH) were analyzed by the colorimetric method. Lipocalin-2 (LCN2) and transforming growth factor-β (TGF-β) were measured using ELIZA. Liver tissues were examined by H&E and Masson trichome staining for detection of liver necrosis or fibrosis. Caspase 3 expression was evaluated by immunohistochemistry. Results: H2S and BM-MSCs ameliorated liver function and inhibited inflammation and oxidative stress detected by significantly decreased serum ALT, AST, ALP, TB, and hepatic MDA, Akt, TGF-β, LCN2, and α-SMA expression and significantly increased CBS and CSE gene expression levels. They attenuated hepatic apoptosis evidenced by decreased hepatic caspase expression.Conclusion: Combined treatment with H2S and BM-MSCs could attenuate liver fibrosis induced by BDL through mechanisms such as anti-inflammation, anti-oxidation, anti-apoptosis, anti-fibrosis, and regenerative properties indicating that using H2S and MSCs may represent a promising approach for management of cholestatic liver fibrosis.
کلیدواژه‌های انگلیسی مقاله Bile duct ligation, Hydrogen sulfide, Liver fibrosis, Mesenchymal stem cells
نویسندگان مقاله | Rehab Ahmed Mohammed
Department of Physiology, Faculty of Medicine, Fayoum University, Egypt


| Heba Mohamed Shawky
Department of Physiology, Faculty of Medicine, Cairo University, Egypt


| Laila Ahmed Rashed
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Egypt


| Hala Mohamed Elhanbuli
Departments of Pathology, Faculty of Medicine, Fayoum University, Egypt


| Dalia Nabil Abdelhafez
Departments of Pathology, Faculty of Medicine, Fayoum University, Egypt


| Eman Sayed Said
Departments of Pharmacology, Faculty of Medicine, Fayoum University, Egypt|Department of Pharmacology and Toxicology, College of Pharmacy, Qassim University, Buraydah 52571, Saudi Arabia


| Ramadan Mostafa Shamardan
Departments of Anatomy, Faculty of Medicine, Fayoum University, Egypt


| Rania Hosny Mahmoud
Departments of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Egypt
نشانی اینترنتی https://ijbms.mums.ac.ir/article_19120.html
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات