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Iranian Journal of Basic Medical Sciences، جلد ۲۴، شماره ۷، صفحات ۹۱۴-۹۲۱
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عنوان انگلیسی Antimicrobial resistance patterns, virulence gene profiles, and genetic diversity of Salmonella enterica serotype Enteritidis isolated from patients with gastroenteritis in various Iranian cities
چکیده انگلیسی مقاله Objective(s): This study aimed to evaluate antibiotic resistance profiles and presence of virulence genes among Salmonella enterica serovar Enteritidis (S. Enteritidis) isolated from patients with gastroenteritis in various regions of Iran. Moreover, genetic relatedness among the strains was assessed by pulsed-field gel electrophoresis (PFGE).Materials and Methods: From April through September 2017, 59 Salmonella strains were isolated from 2116 stool samples. Of these strains, 27 S. Enteritidis were recovered. These strains were subjected to disk diffusion tests, polymerase chain reaction (PCR) for detection of virulence genes (invA, hilA, pefA, rck, stn, ssrA, ssaR, sefA, spvC, sipA, sipC, sopB, sopE, and sopE2), and PFGE.Results: High prevalence of resistance towards cefuroxime (n = 20, 74.1%) and ciprofloxacin (n = 13, 48.2%) were demonstrated. All tested strains possessed invA, hilA, sefA, sipA, sopB, and sopE. The least prevalent virulence gene was rck (n = 6; 22.2%). Based on combinations of virulence genes, 12 virulotypes were observed. The most common virulotype was VP2 (n = 12; 44.4%), harboring all of the virulence genes except for rck. PFGE typing showed only two distinct fingerprints among tested strains. Each fingerprint had completely different virulotypes. Notably, VP4 (harboring all genes except for rck and spvC) was only presented in pulsotype A, while VP2 was confined to pulsotype B.Conclusion: S. Enteritidis strains were derived from a limited number of clones, suggesting that it is highly homogenous. Future works should consider combinations of other genotyping methods together with larger sample sizes from more diverse sources.
کلیدواژه‌های انگلیسی مقاله Antibiotic resistance, Gastroenteritis, PFGE, Salmonella enterica serotype Enteritidis, Virulence genes
نویسندگان مقاله | Fatemeh Fardsanei
Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran


| Mohammad Mehdi Soltan Dallal
Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran|Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran


| Taghi Zahraei Salehi
Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Iran


| masoumeh douraghi
Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran


| Mojtaba Memariani
Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran|Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran


| Hamed Memariani
Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran|Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
نشانی اینترنتی https://ijbms.mums.ac.ir/article_18308.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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