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Iranian Journal of Basic Medical Sciences، جلد ۲۴، شماره ۷، صفحات ۹۷۸-۹۸۵
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عنوان انگلیسی Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons
چکیده انگلیسی مقاله Objective(s): Adrenomedullin (AM) has high expression in the spinal cord. In this study, we investigated the expression of AM and its receptor components, including calcitonin receptor-like receptor (CLR) and receptor activity modifying proteins (RAMPs) in dorsal root ganglion (DRG) and spinal motor (SM) neurons. Furthermore, the effects of AM on cAMP/cAMP response element-binding protein (CREB), AKT/glycogen synthase kinase-3 beta (GSK-3β) signaling pathways, and expressions of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) were evaluated. Materials and Methods: Rat embryonic DRG and SM neurons were isolated, purified, and cultured. Real-time PCR was used to assess expressions of AM, CLR, and RAMPs. cAMP levels, p-CREB, BDNF, and NT-3 were determined using an enzyme-linked immunosorbent assay. p-AKT and p-GSK-3β levels were determined by western blotting. Real-time PCR showed expressions of AM, CLR, RAMP2, and RAMP3 in both DRG and SM neurons. Results: AM increased cAMP accumulation and p-CREB levels in DRG and SM neurons. AM increased p-AKT and p-GSK-3β in DRG, but not SM neurons. AM significantly increased BDNF expression in both DRG and SM neurons. There was also an increase in NT-3 level in both DRG and SM neurons, which is statistically significant in SM neurons. Conclusion: These results showed both DRG and SM neurons are targets of AM actions in the spinal cord. An increase in BDNF expression by AM in both DRG and SM neurons suggests the possible beneficial role of AM in protecting, survival, and regeneration of sensory and motor neurons.
کلیدواژه‌های انگلیسی مقاله Adrenomedullin, Cyclic AMP, Nerve growth factors, Signaling transduction, Spinal Cord
نویسندگان مقاله | Mohsen Sisakht
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran


| Zahra Khoshdel
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran


| Amir Mahmoodzadeh
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran


| Sayed Mohammad Shafiee
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran


| Mohammad Ali Takhshid
Department of Medical Laboratory Sciences, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran|Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
نشانی اینترنتی https://ijbms.mums.ac.ir/article_18397.html
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