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Iranian Journal of Basic Medical Sciences، جلد ۲۴، شماره ۷، صفحات ۹۸۶-۹۹۱
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عنوان انگلیسی Knockout of PKC θ gene attenuates oleic acid-induced acute lung injury via reduction of inflammation and oxidative stress
چکیده انگلیسی مقاله Objective(s): Acute respiratory distress syndrome resulting from acute lung injury has become a momentous clinical concern because of high morbidity and mortality in discharged patients with pulmonary and nonpulmonary diseases. This study aimed to explore the effect of protein kinase C (PKC) θ gene knockout on acute lung injury.Materials and Methods: Wt and PKC θ gene knockout mice were intravenously injected with oleic acid to induce acute lung injury. Pulmonary capillary permeability was assessed via measuring lung wet/dry weight ratio and level of protein in bronchoalveolar lavage fluid (BALF). Histological changes were used to examine acute lung injury. Malondialdehyde (MDA) level, superoxide dismutase (SOD) activity in serum, together with inflammatory cytokines including interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α), were determined. Furthermore, the expressions of heme oxygenase (HO)-1, nuclear factor kappa B (NF κB), and inhibitor of NF-κB alpha (IκB α) were detected in the lungs.Results: PKC θ gene knockout decreased lung wet/dry weight ratio, reduced levels of MDA, IL-6, and TNF-α in serum together with level of protein in BALF. Furthermore, PKC θ gene knockout increased the activities of SOD. Knockout of PKC θ was also observed to increase expression of HO-1 and reduce levels of p-NF κB and p-IKB α in the lungs.Conclusion: These results suggest that PKC θ gene knockout attenuates oleic acid-induced acute lung injury via improving oxidative stress and inflammation.
کلیدواژه‌های انگلیسی مقاله Acute lung injury, Gene knockout, Inflammation, Oleic acid, Oxidative stress, Protein kinase C θ
نویسندگان مقاله | Wei Li
Department of Pathophysiology, Wannan Medical College, Wuhu, China


| Xue Zhao
Department of Pathophysiology, Wannan Medical College, Wuhu, China


| Ting-Ting Yu
Experimental Center for Function Subjects, Wannan Medical College, Wuhu, China


| Wei Hao
Experimental Center for Function Subjects, Wannan Medical College, Wuhu, China


| Guo-Guang Wang
Department of Pathophysiology, Wannan Medical College, Wuhu, China
نشانی اینترنتی https://ijbms.mums.ac.ir/article_18253.html
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