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Iranian Journal of Basic Medical Sciences، جلد ۲۴، شماره ۷، صفحات ۹۹۲-۹۹۶
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عنوان انگلیسی Evaluation of Interleukin-32 and cyclooxygenase-2 expression in HAM/TSP patients and HTLV-1 asymptomatic carriers
چکیده انگلیسی مقاله Objective(s): HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) is a neuroinflammatory disorder associated with HTLV-1. Cytokines and inflammatory mediators have a major role in forming inflammation in HAM/TSP patients. This study aimed to measure the levels of IL-32, a proinflammatory cytokine associated with autoinflammatory disorders, and also cyclooxygenase -2 (COX-2) as a key mediator of inflammatory pathways in HAM/TSP patients and HTLV-1 asymptomatic carriers (ACs).Materials and Methods: Peripheral blood monocyte cells (PBMCs) were isolated from HAM/TSP patients, ACs, and healthy controls (HCs), and DNA and RNA were extracted to evaluate HTLV-1 proviral load (PVL) and expression of IL-32 and COX-2, using real-time PCR. Serum levels of IL-32 were determined by using an ELISA assay.Results: The expression level of IL-32 was significantly higher in ACs compared with HAM/TSP patients and HCs (p 0.05, respectively). There were no statistically significant differences in the expression levels of Cox-2 and protein levels of IL-32 between the study groups. HTLV-1 PVL was higher in HAM/TSP patients compared with ACs.Conclusion: Results showed increased mRNA levels of IL-32 in ACs. Since HTLV-1 PVL in ACs is lower than in HAM/TSP patients, it could be concluded that IL-32 might be an HTLV-1 inhibitor that seems to control virus replication. Despite the difference in IL-32 mRNA levels between study groups, no statistically significant differences were observed in IL-32 serum levels. Also, there were no significant differences in COX-2 expression.
کلیدواژه‌های انگلیسی مقاله Cyclooxygenase-2, HAM/TSP, HTLV-1, Interleukin-32, Inflammation
نویسندگان مقاله | Niayesh Hatatian
Immunology Research Center, Inflammation and Inflammatory Diseases Division, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


| Reza Boostani
Department of Neurology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


| Asadollah Mohammadi
Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran


| Saeedeh Mehraban
Immunology Research Center, Inflammation and Inflammatory Diseases Division, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


| Maryam Mahdifar
Immunology Research Center, Inflammation and Inflammatory Diseases Division, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


| Fariba Zemorshidi
Department of Neurology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


| Sayed-Hamidreza Mozhgani
Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran


| Ali Haji Ghadimi
Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Mohsen Foroughipour
Department of Neurology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


| houshang RafatPanah
Immunology Research Center, Inflammation and Inflammatory Diseases Division, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
نشانی اینترنتی https://ijbms.mums.ac.ir/article_18254.html
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