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Iranian Journal of Basic Medical Sciences، جلد ۲۴، شماره ۶، صفحات ۷۴۴-۷۵۱
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عنوان انگلیسی Time-dependent behavior of the Staphylococcus aureus biofilm following exposure to cold atmospheric pressure plasma
چکیده انگلیسی مقاله Objective(s): Formation of Staphylococcus aureus biofilm leads to persistent infection in tissue or on exter-nal and indwelling devices in patients. Cold atmospheric plasma (CAP) is used for eradication of bacterial biofilms and it has diverse applications in the healthcare system. However, there is not sufficient information on the behavior of biofilms during the CAP exposure period.Materials and Methods: Pre-established S. aureus biofilms were exposed to CAP for 0 to 360 sec, then subjected to washing steps and sonication. Subsequently, biomass, number of colonies, vitality of bacteria, structure of colonies, size of produced particles, and viability of bacteria were evaluated by different assays including crystal violet, colony-forming unit, MTT, scanning electron mi-croscopy, confocal laser scanning microscopy, and dynamic light scattering assays.Results: The results showed that the strength of biomass increased in the first 60 sec, then decreased to less than no-CAP treated controls. Moreover, short CAP exposure (≤60 sec) ehances the fusion of the biofilm extracellular matrix and other components, which results in preservation of bacteria during ultra-sonication and washing steps compared with control biofilms. The S. aureus biofilm structure only breaks down following more CAP exposure (> 90 sec) and demolition. Interestingly, the 60 sec CAP exposure could cause the fusion of biofilm compo-nents, and large particles are detectable. Conclusion: According to this study, an inadequate CAP exposure period prevents absolute eradication of biofilm and enhances the preservation of bacteria in stronger biofilm compartments.
کلیدواژه‌های انگلیسی مقاله Anti-infective agents, Biofilm, Extracellular polymeric substances, Non-thermal atmospheric pressure plasma, Staphylococcus aureus
نویسندگان مقاله | Foad Fahmide
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran


| Parastoo Ehsani
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran


| Mohammad Atyabi
Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran
نشانی اینترنتی https://ijbms.mums.ac.ir/article_18125.html
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زبان مقاله منتشر شده en
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