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Iranian Journal of Basic Medical Sciences، جلد ۲۴، شماره ۶، صفحات ۸۰۵-۸۱۴
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عنوان انگلیسی Therapeutic potential of mesenchymal stem cells for peripheral artery disease in a rat model of hindlimb ischemia
چکیده انگلیسی مقاله Objective(s): Mesenchymal stem cells are viewed as the first choice in regenerative medicine. This study aimed to elucidate the influence of BM-MSCs transplantation on angiogenesis in a rat model of unilateral peripheral vascular disease. Materials and Methods: Twenty-one rats were arbitrarily allocated into three groups (7/group). Group I: control sham-operated rats, Group II: control ischemic group: Rats were subjected to unilateral surgical ligation of the femoral artery, and Group III: ischemia group: Rats were induced as in group II, 24 hr after ligation, they were intramuscularly injected with BM-MSCs. After scarification, gastrocnemius muscle gene expression of stromal cell-derived factor-1 (SDF-1), CXC chemokine receptor 4 (CXCR4), vascular endothelial growth factor receptor 2 (VEGFR2), von Willebrand factor (vWF), and hypoxia-inducible factor-1α (HIF-1α) were analyzed by quantitative real-time PCR. Muscle regeneration and angiogenesis evaluation was assessed through H&E staining of the tissue. Furthermore, Pax3 and Pax7 nuclear expression was immunohistochemically assessed. Results: Rats treated with BM-MSCs showed significantly raised gene expression levels of SDF-1, CXCR4, VEGFR2, and vWF compared with control and ischemia groups. H&E staining of the gastrocnemius showed prominent new vessel formation. Granulation tissue within muscles of the ischemic treated group by BM-MSCs showed cells demonstrating nuclear expression of Pax3 and Pax7. Conclusion: BM-MSCs transplantation has an ameliorating effect on muscle ischemia through promoting angiogenesis, detected by normal muscle architecture restoration and new blood vessel formations observed by H&E, confirmed by increased gene expression levels of SDF-1, CXCR4, VEGFR2, and vWF, decreased HIF-1α gene expression, and increased myogenic Pax7 gene expression.
کلیدواژه‌های انگلیسی مقاله CXC chemokine receptor 4, Mesenchymal stem cells, Peripheral vascular disease, Vascular endothelial growth factor receptor 2, Von Willebrand factor
نویسندگان مقاله | Amani Ali
Department of Medical Physiology, Faculty of Medicine, Fayoum University, Fayoum, Egypt


| Amira Ahmed
Hormones Department, Medical Research Division, National Research Centre, Giza, Egypt


| Dina El-Yasergy
Department of Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt


| Moustafa Abousarie
Department of Pathology, Faculty of Medicine, Fayoum University, Fayoum, Egypt


| Ramadan Elsayed
Department of Medical Anatomy, Faculty of Medicine, Fayoum University, Fayoum, Egypt


| Yasmin Mohammed
Department of Medical Anatomy, Faculty of Medicine, Fayoum University, Fayoum, Egypt


| Rahab Mohammed
Department of Medical Physiology, Faculty of Medicine, Fayoum University, Fayoum, Egypt
نشانی اینترنتی https://ijbms.mums.ac.ir/article_18023.html
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