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Iranian Journal of Basic Medical Sciences، جلد ۲۴، شماره ۵، صفحات ۶۷۵-۶۸۱
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عنوان انگلیسی Sirtuin 3 deficiency promotes acute kidney injury induced by sepsis via mitochondrial dysfunction and apoptosis
چکیده انگلیسی مقاله Objective(s): To explore the regulation mechanism of Sirtuin 3 (SIRT3) on the mitochondrial function and apoptosis of acute kidney injury (AKI) in septic mice.Materials and Methods: The sepsis-induced AKI model was constructed in the wild-type and SIRT3 knockout (KO) mice, and the levels of serum creatinine (Scr) and plasma kidney injury molecule 1 (pKIM-1) in mice were detected by ELISA. The mitochondrial damage of kidney tubular epithelial cells (KTEC) was observed by electron microscopy, the apoptosis of KTEC was detected by TUNEL assay, and the mRNA levels of SIRT3, Bax, Caspase-3, and Bcl-2 were detected by RT-qPCR.Results: SIRT3 KO caused increased expression of Scr, pKIM-1, and inducible nitric oxide synthase protein in the kidneys of septic mice, and decreased the levels of superoxide dismutase, catalase, and mitochondrial complex enzymes I/II/III/IV. SIRT3 deficiency exacerbated histopathological and mitochondrial damage to the proximal tubules of the kidney. In addition, SIRT3 KO resulted in a significantly increased apoptosis of KTEC, increased the mRNA levels of Bax and Caspase-3, and decreased the mRNA levels of Bcl-2. Conclusion: Our study suggests that SIRT3 deficiency promotes sepsis-induced AKI via increasing oxidative stress, mitochondrial dysfunction, and inducing apoptosis.
کلیدواژه‌های انگلیسی مقاله Acute kidney injury, Apoptosis, Mitochondria, Sepsis, Sirtuin3
نویسندگان مقاله | Heng Fan
Department of Intensive Care Unit, Ningbo First Hospital, Ningbo, Zhejiang Province, P.R China


| Jian-wei Le
Department of Intensive Care Unit, Ningbo First Hospital, Ningbo, Zhejiang Province, P.R China


| Min Sun
Department of Intensive Care Unit, Ningbo First Hospital, Ningbo, Zhejiang Province, P.R China


| Jian-hua Zhu
Department of Intensive Care Unit, Ningbo First Hospital, Ningbo, Zhejiang Province, P.R China
نشانی اینترنتی https://ijbms.mums.ac.ir/article_17819.html
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