این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Basic Medical Sciences، جلد ۲۴، شماره ۳، صفحات ۳۷۷-۳۸۲
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Immunogenicity evaluation of the HIV-1 Tat containing polyepitope DNA vaccine adjuvanted with CpG-ODNs in mice
چکیده انگلیسی مقاله Objective(s): HIV-1 is still considered a serious threat to human health, and accessibility of a suitable and efficient vaccine is urgently needed to address the disease burden. DNA vaccines employ the cells of the vaccinated hosts for in situ production of the vaccines. This strategy is an alternative and effective approach for traditional vaccination against high-risk pathogens, e.g., HIV-1. On the other hand, polyepitope vaccines, containing several immunogenic and conserved epitopes from virus vital regulatory and structural proteins, could more efficiently induce cellular and humoral immune responses against different clades of the virus. Materials and Methods: Herein, we comparatively investigated the immunogenic potency of the HIV-1 polytope DNA vaccine containing CpG oligodeoxynucleotides (CpG-ODNs) in BALB/c mice. To this end, after verifying the expression of the recombinant sequence in the eukaryotic HEK 293 cell line, it was amplified and extracted in the prokaryotic host cells (E. coli DH5α)) and then formulated and administered intramuscularly (IM) to the experimental mice (on days 0, 14, and 28) with and without CpG-ODNs adjuvant. Results: Taken together, the results demonstrated that CpG-ODNs adjuvanted DNA vaccine could significantly elicit cellular and humoral immune responses in the immunized animals in comparison with the control ones (p < 0.05). Conclusion: Regarding the obtained results and also considering the advantages of polytopic and DNA vaccines, this approach might be considered a new regimen in HIV-1/AIDS vaccination.
کلیدواژه‌های انگلیسی مقاله Cellular immunity CpG oligodeoxynucleotides DNA vaccine HIV, 1 Humoral immunity
نویسندگان مقاله | Ehsan Jazaeri
Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, Iran


| Atiyeh Mahdavi
Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, Iran


| Asghar Abdoli
Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran
نشانی اینترنتی https://ijbms.mums.ac.ir/article_17608.html
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات