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Iranian Journal of Basic Medical Sciences، جلد ۲۴، شماره ۳، صفحات ۳۸۳-۳۹۰
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عنوان انگلیسی Effects of self-assembled cell-penetrating peptides and their nano-complexes on ABCB1 expression and activity
چکیده انگلیسی مقاله Objective(s): Doxorubicin (Dox) is one of the most well-known chemotherapeutics that are commonly applied for a wide range of cancer treatments. However, in most cases, efflux pumps like P-glycoprotein (P-gp), expel the taken drugs out of the cell and decrease the Dox bioavailability. Expression of P-gp is associated with elevated mRNA expression of the ATP-binding cassette B1 (ABCB1) gene. Materials and Methods: In the current study, different sequences of cell-penetrating peptides (CPPs) containing tryptophan, lysine, and arginine and their nano-complexes were synthesized and their impact on the expression and activity of the ABCB1 gene was evaluated in the A549 lung carcinoma cell line. Furthermore, the cellular uptake of designed CPPs in the A549 cell line was assessed. Results: The designed peptides, including [W4K4], [WR]3-QGR, R10, and K10 increased Dox cytotoxicity after 48 hr. Furthermore, arginine-rich peptides showed higher cellular uptake. Rhodamin123 accumulation studies illustrated that all the obtained peptides could successfully inhibit the P-gp pump. The designed peptides inhibited the ABCB1 gene expression, of which, [W4K4] resulted in the lowest expression ratio. Conclusion: [W4K4], [WR]3-QGR, R10, and K10 could successfully increase the Dox cytotoxicity by decreasing the efflux pump gene expression.
کلیدواژه‌های انگلیسی مقاله Cancer therapy CPPs Doxorubicin Multi, drug resistance P, gp
نویسندگان مقاله | Mehri Niazi
Student Research Committee, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran|Drug Applied Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran


| Parvin Zakeri-Milani
Liver and Gastrointestinal Diseases Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran


| Mehdi Soleymani-Goloujeh
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran


| Ali Mohammadi
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran


| Muhammad Sarfraz
College of Pharmacy, Al Ain University of Science and Technology, Al Ain 64141, UAE


| Raimar Löbenberg
Faculty of Pharmacy and Pharmaceutical Sciences, Katz Group Centre for Pharmacy and Health Research, University of Alberta, Edmonton, Alberta, T6G 2H5, Canada


| saeedeh Najafi-Hajivar
Student Research Committee, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran


| Javid Shahbazi Mojarrad
Biotechnology Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran


| Masoud Farshbaf
Student Research Committee, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran


| Hadi Valizadeh
Drug Applied Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
نشانی اینترنتی https://ijbms.mums.ac.ir/article_17578.html
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