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Iranian Journal of Basic Medical Sciences، جلد ۲۴، شماره ۳، صفحات ۴۰۰-۴۰۷
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عنوان انگلیسی Correlation between antibiotic resistance and phylogenetic types among multidrug-resistant Escherichia coli isolated from urinary tract infections
چکیده انگلیسی مقاله Objective(s): Emergence of multidrug resistance has reduced the choice of antimicrobial regimens for UTIs. To understand the association of phenotype and genotype among uropathogens.Materials and Methods: Six hundred and twenty-eight (628) urine samples were collected and analyzed. Antibiotic sensitivity pattern was determined by the Kirby-Bauer Disc Diffusion Method and minimum inhibitory concentration (MIC) was tested by the E test. Fluoroquinolone resistant mutations in QRDR of gyrA and ParC, phylogenetic groups, and PAIusp subtype were detected by PCR.Results: Most prevalent uropathogens were Escherichia coli (53.2%) followed by Klebsiella pneumoniae (21%). Multidrug- resistance was observed in > 50% cases for third-generation cephalosporins and ciprofloxacin and lowest in meropenem. E. coli (66.2%) and K. pneumonia (64.4%) were extended-spectrum β-lactamases (ESBLs) producers. MIC to trimethoprim-sulfamethoxazole was highest in E. coli (>1024 µg/ml). In 80 (24%) of the 334 E. coli isolates analyzed in detail, 54 fluoroquinolones (FQ) resistant isolates carried mutations (S83L, D87N, S80I, E84V) in QRDR of gyrA and ParC. Out of 54 FQ-resistant isolates, 43 (79.6%) isolates belonged to the phylogenetic group B2, and 11(20.4%) belonged to group D. Isolates belonged to group B2, 38 (88.4%) of the 43 isolates carried PAIusp subtype IIa and high frequency of mutation E84V in ParC was detected in 37 (97.4%). Other mutations, such as S80I, S83L in gyrA and D87N in ParC were found in all resistant isolates.Conclusion: Correlations between phenotype and genotype provided a basis to understand the resistance development in uropathogens, and PAIusp subtyping indicated that E. coli belonged to the B2 group.
کلیدواژه‌های انگلیسی مقاله Escherichia coli, ESBL, MDR, PCR, UTI
نویسندگان مقاله | Humera Nazir
Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan|Institute of Pure and Applied Biology, (Microbiology division) Bahauddin Zakariya University, Multan 60900, Pakistan


| Mubashar Aziz
Institute of Pure and Applied Biology, (Microbiology division) Bahauddin Zakariya University, Multan 60900, Pakistan


| Zulfiqar Mirani
Department of Microbiology, PCSIR Laboratories Complex, Karachi 74000, Pakistan


| Ahsan Sheikh
Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan


| Muhammad Saeed
Institute of Pure and Applied Biology, (Microbiology division) Bahauddin Zakariya University, Multan 60900, Pakistan


| Masroor Hussain
Department of Biotechnology, University of Science & Technology Bannu, Pakistan.


| Aleem Khan
Institute of Pure and Applied Biology, (Microbiology division) Bahauddin Zakariya University, Multan 60900, Pakistan


| Tahira Ruby
Department of Zoology, The Islamia University of Bbahawalpur,61300, Bahawalpur, Pakistan


| Naseem Rauf
PCSIR Laboratories Islamabad, Pakistan
نشانی اینترنتی https://ijbms.mums.ac.ir/article_17610.html
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