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Iranian Journal of Basic Medical Sciences، جلد ۲۳، شماره ۱۱، صفحات ۱۴۳۹-۱۴۴۴
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عنوان انگلیسی In vivo therapeutic effects of colorectal cancer cell-derived exosomes
چکیده انگلیسی مقاله Objective(s): Exosomes are nano-sized structures with lipid bilayer membranes that can be secreted by cancer cells. They play an important role in the biology of the tumor extracellular matrix. Exosomes may contain and transfer tumor antigens to dendritic cells to trigger T cell-mediated anti-tumor immune responses. Materials and Methods: BALB/c mice bearing CT26 colorectal cancer were treated subcutaneously with purified exosomes from analogous tumor cells. The mice were analyzed with respect to tumor size, survival, and anti-tumor immunity responses, including gene expression of cytokines and flowcytometry analysis of T lymphocytes. Results: The rate of tumor size growth in the exosome-treated group significantly decreased (p < 0.05), and the flow cytometry results showed a significant reduction in the spleen regulatory T cells (Tregs) count of the exosome-treated group, compared with the untreated group (P=0.02). Although the increase in the serum level of interferon-γ (IFN-γ) and the number of cytotoxic CD8 T lymphocytes (CTLs) in the spleen tissue was not significant (P>0.05), the gene expression of IFN-γ increased significantly (P=0.006). Conclusion: The present results revealed that subcutaneous administration of tumor-derived exosomes could effectively lead to the inhibition of tumor progression by decreasing the number of Treg cells and up-regulation of the IFN-γ gene. Therefore, tumor-derived exosomes can be used as potential vaccines in cancer immunotherapy.
کلیدواژه‌های انگلیسی مقاله CT26 colorectal cancer cells Cytotoxic T, Lymphocyte Exosome Interferon, gamma Tumor, derived exosomes
نویسندگان مقاله | Ali Ganji
Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran|Department of Microbiology and Immunology, School of Medicine, Arak University of Medical Sciences, Arak, Iran


| Iman Farahani
Department of Microbiology and Immunology, School of Medicine, Arak University of Medical Sciences, Arak, Iran


| Mana Shojapuor
Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran


| Ali Ghazavi
Department of Microbiology and Immunology, School of Medicine, Arak University of Medical Sciences, Arak, Iran|Traditional and Complementary Medicine Research Center (TCMRC), Arak University of Medical Sciences, Arak, Iran


| Ghasem Mosayebi
Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran|Department of Microbiology and Immunology, School of Medicine, Arak University of Medical Sciences, Arak, Iran
نشانی اینترنتی https://ijbms.mums.ac.ir/article_16577.html
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