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Iranian Journal of Microbiology، جلد ۱۴، شماره ۱، صفحات ۱۱۹-۱۲۴
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عنوان انگلیسی Investigation of human parvovirus B19 prevalence in a large healthy umbilical cord blood donors
چکیده انگلیسی مقاله Background and Objectives: Umbilical cord blood (UCB) was used to source hematopoietic stem cells in the past. Despite the apparent advantages of UCB transplantation, virus reactivation poses a considerable danger in allogeneic hematopoietic stem cell transplantation (HSCT). Human Parvovirus B19 is regarded as a potential threat to UCB contamination. This study aimed to evaluate the prevalence of parvovirus B19 in cord blood donors by Semi-Nested PCR. This study is the first large-scale report of the B19 DNA in cord blood donors in Iran. Materials and Methods: A total of 691 umbilical cord blood were collected under standard procedure. Then, DNA from buffy coat and plasma were extracted, and semi-nested PCR was performed for all samples. Results: Two out of 691 samples (0.29%) indicated viremia in plasma and buffy coat. Conclusion: In this line, designing and validating a quantitative PCR assay for detection, quantification, and discrimination of Human B19 DNA genotypes of cord blood donors is necessary to enhance the safety of this source of stem cells.
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نویسندگان مقاله | Hooman Ramezany
Department of Immunology, Research Center of Blood Transfusion, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran


| Maryam Kheirandish
Department of Immunology, Research Center of Blood Transfusion, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran


| Shahram Samiee
Department of Immunology, Research Center of Blood Transfusion, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran


| Mina Khosravifar
Non-Communicable Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran


| Seyedeh Melika Hashemi
Non-Communicable Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/3426
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