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Iranian Journal of Microbiology، جلد ۱۳، شماره ۲، صفحات ۲۰۴-۲۱۱
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عنوان انگلیسی Expression of type II toxin-antitoxin systems and ClpP protease of methicillin-resistant Staphylococcus aureus under thermal and oxidative stress conditions
چکیده انگلیسی مقاله Background and Objectives: Staphylococcus aureus is a main human pathogen that causes a variety of chronic to persistent infections. Across the diverse factors of pathogenesis in bacteria, Toxin-Antitoxin (TA) systems can be considered as an antibacterial target due to their involvement in cellular physiology counting stress responses. Here, the expression of TA system genes and ClpP protease was investigated under the thermal and oxidative conditions in S. aureus strains. Materials and Methods: The colony-forming unit (CFU) was used to determine the effects of thermal and oxidative stresses on bacterial survival. Moreover, the expressions of TA system genes in S. aureus strains were evaluated 30 min and 1 h after thermal and oxidative stresses, respectively, by quantitative reverse transcriptase real-time PCR (qRT-PCR). Results: The cell viability was constant across thermal stress while oxidative stress induction showed a significantly decrease in the growth of Methicillin-Resistant S. aureus (MRSA) strain. Based on the qRT-PCR results, the expression of mazF gene increased under both thermal and oxidative stresses in the MRSA strain. Conclusion: A putative TA system (namely immA/irrA) most likely has a role under the stress condition of S. aureus. The MRSA strain responds to stress by shifting the expression level of TA genes that has diverse effects on the survival of the pathogen due to the stress conditions. The TA systems may be introduced as potential targets for antibacterial treatment.
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نویسندگان مقاله | Samira Karimaei
Department of Pathobiology, Biotechnology Research Center, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Behrooz Sadeghi Kalani
Department of Medical Microbiology, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran; Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran


| Nader Shahrokhi
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran


| Rahil Mashhadi
Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran


| Mohammad Reza Pourmand
Department of Pathobiology, Biotechnology Research Center, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/2874
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