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Iranian Journal of Microbiology، جلد ۱۳، شماره ۲، صفحات ۲۳۵-۲۴۲
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عنوان انگلیسی Immunological detection of AcAMP antimicrobial peptide secreted by Aspergillus clavatus
چکیده انگلیسی مقاله Background and Objectives: Aspergillus clavatus antimicrobial peptide (AcAMP) is a fungi-derived peptide with a broad spectrum of activity against pathogenic bacteria and fungi. Natural antimicrobial peptides, including AcAMP, have attracted many attentions in the development of new natural antibiotics against pathogenic bacteria, especially multidrug resistant ones. Materials and Methods: In the present study, acamp gene was codon-optimized and chemically synthesized in pUC57 cloning vector, subcloned into pET28a (+) expression vector and transferred into competent Escherichia coli BL21 (DE3) cells. The expression of AcAMP was induced by addition of Isopropyl β- d-1-thiogalactopyranoside (IPTG) and the expressed peptide was purified by Ni-NTA. BALB/c mice were immunized with the purified peptide and the ability of the immunized mice sera for the detection of the native AcAMP secreted by A. clavatus IRAN 142C was examined through ELISA and Western blotting techniques. Results: Both ELISA and Western blotting demonstrated the ability of the sera of the immunized mice to detect the native AcAMP. Conclusion: The results of the present work show that the raised antibody against recombinant AcAMP can be used to detect AcAMP peptide, an issue which paves the way to develop detection kits for the detection of AcAMP-producing organisms, purification of this valuable peptide for further investigations.
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نویسندگان مقاله | Ehsan Zamani
Department of Biological Sciences, Faculty of Sciences, Imam Hossein University, Tehran, Iran


| Jamil Zargan
Department of Biological Sciences, Faculty of Sciences, Imam Hossein University, Tehran, Iran


| Hossein Honari
Department of Biological Sciences, Faculty of Sciences, Imam Hossein University, Tehran, Iran


| Abbas Hajizade
Department of Biological Sciences, Faculty of Sciences, Imam Hossein University, Tehran, Iran


| Ashkan Haji Noor Mohammadi
Department of Biological Sciences, Faculty of Sciences, Imam Hossein University, Tehran, Iran


| Hani Keshavarz Alikhani
Department of Biology, Razi University, Kermanshah, Iran


| Ahmad Heidari
Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Zanjan, Iran


| Mohammad Hossein Pour
Department of Biological Sciences, Faculty of Sciences, Imam Hossein University, Tehran, Iran
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/2597
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