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Research in Pharmaceutical Sciences، جلد ۱۶، شماره ۲، صفحات ۱۲۹-۱۴۰
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی The effects of Benjakul extract and its isolated compounds on cell cycle arrest and apoptosis in human non-small cell lung cancer cell line NCI-H226
چکیده انگلیسی مقاله Background and purpose: Benjakul, a traditional Thai formulation for cancer treatment, is composed of five plants. This study aimed to assess the cytotoxicity of Benjakul, its five plants, and its isolated compounds against non-small cell lung cancer (NSCLC) by the sulforhodamine B (SRB) assay. Experimental approach: Analyses of cell cycle and membrane asymmetry changes were performed with different fluorescent dyes and analyzed by flow cytometry in NCI-H226 cells. Activation of caspase-3 was measured using a caspase-3 colorimetric assay kit. The pan-caspase inhibitor Z-VAD-FMK was used in analyses of cell cycle and caspase-3 activity. Findings/Results: Benjakul exhibited cytotoxicity against NSCLC with IC 50 between 5.56-5.64 μg/mL. Among its five ingredients, Benjakul displayed the highest selectivity with selectivity index values ranging from 2.93 to 6.88, with the exception of Plumbago indica , indicating its protective effects. Plumbagin and 6-shogaol displayed the highest cytotoxicity and underwent molecular studies in NCI-H226 cells. Flow cytometry analysis revealed that Benjakul and 6-shogaol dose-dependently induced G2/M phase arrest, and plumbagin dose-dependently induced S-G2/M phase arrest with the highest percentage in early incubation time (12-24 h). At the highest doses, Benjakul extract, 6-shogaol, and plumbagin time-dependently increased the population of sub-G1 apoptotic cells with the highest percentage in longer incubation time (60-72 h). Similarly, membrane asymmetry changes showed time-dependent increases in the percentage of early and late apoptotic cells. Moreover, the apoptosis-inducing effect of Benjakul, 6-shogaol, and plumbagin at the highest dose, via the caspase cascade was confirmed by time-dependent induction of caspase-3 activity, followed by its complete reduction and abolished sub-G1 peaks upon addition of Z-VAD-FMK. Conclusion and implication: Our findings demonstrated for the first time the effects of Benjakul and its compounds on S-G2/M or G2/M phase arrest and caspase-dependent apoptosis in lung cancer cells.
کلیدواژه‌های انگلیسی مقاله Apoptosis,Benjakul,Cytotoxic activity,Plumbagin,6-Shogaol.
نویسندگان مقاله | Arunporn Itharat
3Faculty of Pharmacy, Mahasarakham University, Kantarawichai, Maha Sarakham 44150, Thailand.


| Ruchilak Rattarom
2Center of Excellence in Applied Thai Traditional Medicine Research (CEATMR), Thammasat University, Klong Luang, Pathumthani 12120, Thailand. 4Department of Preclinical Science, Faculty of Medicine, Thammasat University, Klong Luang, Pathumthani 12120, Thailand.


| Pintusorn Hansakul
1Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Klong Luang, Pathumthani 12120, Thailand. 2Center of Excellence in Applied Thai Traditional Medicine Research (CEATMR), Thammasat University, Klong Luang, Pathumthani 12120, Thailand.


| Intouch Sakpakdeejaroen
5Department of Agricultural Food and Nutritional Science, Faculty of Agricultural Life and Environmental Sciences, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.


| Buncha Ooraiku
6Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Canada.


| Neal M. Davies


نشانی اینترنتی http://rps.mui.ac.ir/index.php/jrps/article/view/2050
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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