| کلیدواژههای انگلیسی مقاله |
Ornithogalum bungei Boiss, Hyacinthaceae, Biological products, HepG2, PC3, K562 cells, What&,rsquo s Known Natural products are good sources for the development of novel cytotoxic agents. In Iran, Ornithogalum bungei Boiss (Hyacinthaceae) is an endemic plant with different biological activities. There are findings about the medicinal background of Ornithogalum bungei. What&,rsquo s New For the first time, the cytotoxic activity of different parts of Ornithogalum bungei Boiss extracts and fractions on different cell lines of hepatocarcinoma (HepG2), prostate cancer (PC3), and chronic myelogenous leukemia (K562) were investigated. Ornithogalum bungei dichloromethane extract and fractions showed potent cytotoxic activity on HepG2 and PC3 cells. IntroductionCancer is known to be one of the significant public health problems in most countries and is considered as one of the leading causes of death. 1, Medicinal plants have historically been used to treat various diseases in different parts of the world. 2, The main reason behind their use in medical aspects is their secondary metabolites production. 3, Natural products have been recognized as potential sources for anti-cancer candidate molecules. Over half of all the pharmaceuticals or new chemical entities entering the market or clinical trials are still of natural origin. 4, Ornithogalum L. genus belongs to the subclass Monocotyledonae and is classified in the Hyacinthaceae family. Certain species of the Ornithogalum genus have exhibited anti-cancer and anti-inflammatory effects in Chinese folk medicine. 5, Although here are a few findings concerning the folkloric medicinal background, some other species are used in floriculture and even gardens as cut flowers. 6, In addition, a previous report showed some poisonous species. 7, Several cardenolide glycosides have been isolated from a number of species of the Ornithogalum genus. In 2012, Mimaki and colleagues isolated a cholestane glycoside OSW-1 (3&,beta ,16&,beta ,17&,alpha -trihydroxycholest-5-en-22-one16-O-(2-O-4-methoxybenzoyl-&,beta -D-xylopyranosyl)-(1&,rarr 3)-(2-O acetyl-&,alpha -L-arabinopyranoside)) from the bulbs of O. saundersiae with significant cytostatic activities against various malignant tumor cells, such as HL-60 (human promyelocytic leukemia) and MOLT-4 (human T lymphoblast acute lymphoblastic leukemia) through the induction of apoptosis. 8, - 11, Another investigation on the bulbs of O. saundersiae demonstrated some rearranged cholestane glycosides with a glycosidic moiety at C-16. These important findings resulted in the synthesis of OSW-1 and its active analog, which acts by damaging the mitochondria and apoptosis induction through an increase in cytosolic calcium. 12, , 13, In addition, investigation on the ethanol (EtOH) extract of O. saundersiae showed its protective effect against acute hepatic failure by apoptosis of hepatocytes and anti-inflammatory activity. 13, Some other studies on O. saundersiae presented the OSW1 activity against some resistant anti-cancer agents, such as fludarabine-resistant chronic lymphocytic leukemia cells. 14, , 15, The aerial parts of O. cuspidatum were used as a food additive and sore throat calming agent in Iranian traditional medicine. The flowers and bulbs of O. cuspidatum have been reported to have saturated hydrocarbons, and the leaves are believed to contain oxygenated hydrocarbons as well as terpenoid compounds. 16, Bio-screening investigations of the methanol (MeOH) extract of the bulbs of O. thyrsoides introduced high to moderate cytotoxic cholestane bisdesmosidic, spirocyclic glycosides, and cholestane glycosides against leukemia HL-60 cells. 17, , 18, In the present work, the anti-cancer property of MeOH extract obtained from the shoots of O. narbonense was investigated on human colon cancer (DLD-1), endometrium cancer (ECC-1), and embryonic kidney cancer (HEK-293) cells. The results indicated that O. narbonense extract caused an increase in the amount of intracellular free radicals leading to associated DNA damage as well as concentration-related apoptosis in cancer cells. 19, Ornithogalum bungei Boiss., which is locally called &,ldquo Shir-morgh&,rdquo , is an endemic species distributed in the Golestan forests of Iran. 20, , 21, Flowers of this bulbous plant are white with a green stripe on the midrib of the petals. 22, The biological activities of O. bungei have not been reported in previous papers. Thanks to pharmacological screening, such as cytotoxic activity investigations, scientists could obtain important preliminary data to select plant extracts with potential anti-tumor properties. This study aimed to evaluate the anti-tumor effects of the extracts and fractions from different parts of O. bungei on HepG2, PC3, and K562 cell lines. Materials and MethodsThe Ethics Committee of Tehran University of Medical Sciences approved the study protocol under the code IR.TUMS.PSRC.REC.1396.2242, Tehran, Iran. The experiments were performed at the School of Pharmacy, Tehran University of Medical Sciences (Tehran, Iran), during 2017-2019. Plant Collection The plants were collected by the authors in May 2016 from Golestan province, Iran. Taxonomic identification was carried out scientifically, and a voucher specimen has been deposited at the herbarium of the School of Sciences, University of Tehran (voucher number, 48680-TUH). Extraction Different parts of O. bungei, including bulbs, stems, flowers, fruits, and seeds, were carefully separated, air-dried at room temperature, and grounded by a mill into fine powder. The extraction was performed stepwise with dichloromethane (DCM) and MeOH in triplicate according to the following procedure, the dried plant samples were placed in separate flasks, mixed with proper amounts of solvent, sonicated in an ultrasonic water bath at 40 &,deg C for 30 minutes, and then mechanically stirred for 24 hours (three times). The solutions of the obtained extract were filtered and concentrated under a vacuum using a rotary evaporator at 40 &,deg C (table 1,).SubstanceBulbStemFlowerFruitSeedDried material (g)124.01172.0053.0020.005.00DCM extract (g)2.7331.3212.000.810.38MeOH extract (g)24.00131.2020.003.340.42DCM extract, Dichloromethane extract MeOH extract, Methanol extract |
| نویسندگان مقاله |
Paria Sharafi-Badr |
Department of Pharmacognosy, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Sepideh Karoobi |
Department of Toxicology and Pharmacology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Hamid Reza Monsef-Esfahani |
Department of Pharmacognosy, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Mohammad Hossein Ghahremani |
Department of Toxicology and Pharmacology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Hamid-Reza Adhami |
Department of Pharmacognosy, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
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