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Basic and Clinical Cancer Research، جلد ۱۲، شماره ۳، صفحات ۱۱۲-۱۱۹
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عنوان انگلیسی The Effect of Piperine on MMP-9, VEGF, and E-cadherin Expression in Breast Cancer MCF-7 Cell Line
چکیده انگلیسی مقاله Background: Vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9), and E-cadherin play a vital role in the behavior of angiogenesis, metastasis, and invasion of breast tumor cells. Piperine, the main component of Piper Nigrum, has shown anti-cancer properties in various malignancies. This Study investigates the potential effect of piperine on MMP-9, E-cadherin, and VEGF expression in breast cancer MCF-7 cell line. Methods: MTT assay was applied to assess the viability of MCF-7 cells. The mRNA levels of MMP-9, VEGF, and E-cadherin were assayed by qRT-PCR. Western blot was performed to identify the protein level of MMP-9. Results: MTT assay results showed that piperine treatment (5, 10, 25, 50, 75, and 100 μM) for 24 hours effectively inhibited cell viability of MCF-7 cells as compared with the control group. Furthermore, the gene expression of VEGF, MMP-9, and E-cadherin was dose-dependently suppressed by piperine treatment (5, 10 and 25 μM) (P< 0.05; P< 0.01). The results also indicated that piperine (5, 10, and 25 μM) significantly suppressed MMP-9 protein expression after 24 hours of piperine treatment (P< 0.01).   Conclusion: These results suggest that piperine may prevent angiogenesis, migration, and invasion of MCF-7 cells by suppressing MMP-9 and VEGF, and by inducing E-cadherin expression. Hence, it may be a suitable candidate for designing new drugs in cancer therapy.
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نویسندگان مقاله | Zahra Zare
Department of Biology, Farhangian University, Tehran, Iran.


| Tina Nayerpour Dizaj
Department of Medical Biotechnology, Faculty of Modern Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.


| Armaghan Lohrasbi
Department of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA, UK.


| Zakieh Sadat Sheikhalishahi
Department of Physiology, Shahid Sadoghi University of Medical Sciences, Yazd, Iran.


| Mohammad Panji
Department of Cell and Molecular Biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran.


| Fahimeh Hosseinabadi
Biology Department, Faculty of Sciences, Arak University, Arak, Iran.


| Vajiheh Najafi
Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran.


| Omid Abazari
Department of Clinical Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.


| Mojtaba Abbasi
Veterinary Medicine, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.


| Parisa Khanicheragh
Department of Clinical Biochemistry, Lorestan University of Medical Sciences, Khorramabad, Iran.
نشانی اینترنتی https://bccr.tums.ac.ir/index.php/bccrj/article/view/349
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