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Medical Laboratory Journal، جلد ۱۶، شماره ۱، صفحات ۲۵-۳۱
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Phytochemical Analysis of Garlic Hydro-alcoholic Extract and Evaluation of its Anti-bacterial Effect on Enterohemorrhagic Escherichia coli in Vitro and ex Vivo
چکیده انگلیسی مقاله Background and objectives: Garlic is a medicinal plant with various health promoting properties including antimicrobial effects. In this study, we investigated in vitro antibacterial effects of garlic hydro-alcoholic extract against enterohemorrhagic Escherichia coli (EHEC). Methods: Garlic hydro-alcoholic extract was prepared by maceration method.  Phytochemical analysis of the extract was carried out using gas chromatography-mass spectrometry. Minimum inhibitory concentration (MIC) of the extract against EHEC was determined by micro-dilution assay. Cytotoxic effect of the garlic extract on human colon adenocarcinoma cell line (SW480) was assessed using MTT assay. Micro-dilution assay was also used to determine the MIC of the extract against EHEC when co-cultured with SW480 cells. Results: The amount of organosulfur in garlic extract was 70.91% and the most common organosulfur compounds were trisulfide, di-2-propenyl (34.8%) and diallyl disulfide (14.83%). The MIC of garlic hydro-alcoholic extract on EHEC alone and when co-cultured with SW480 was 12.5 mg/ml. Concentrations of 12.5 mg/ml and 25 mg/ml of the extract significantly reduced the viability of SW480 cells compared to control and concentration of 6.25 mg/ml of garlic extract (p < 0.0001). Conclusion: The garlic hydro-alcoholic extract has inhibitory effects on EHEC in vitro. Therefore, it can be considered a suitable candidate for controlling infections caused by EHEC.
کلیدواژه‌های انگلیسی مقاله Cell line,Plant extracts,Garlic
نویسندگان مقاله | Zynab badeli
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran


| Masoud Haghkhah
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran


| Ezzat Allah Ghaemi
Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran
نشانی اینترنتی http://mlj.goums.ac.ir/browse.php?a_code=A-10-826-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده میکروب شناسی
نوع مقاله منتشر شده Research Article
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