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Journal of Health Sciences and Surveillance System، جلد ۱۰، شماره ۱، صفحات ۱۲۶-۱۳۳
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عنوان انگلیسی Optimization of Col H Gene Encoding Clostridium histolyticum Collagenase to Express in Escherichia coli
چکیده انگلیسی مقاله Background: A given amino acid sequence can be encoded by a huge number of different nucleic acid sequences. These sequences, however, have proved not to be equally useful. The choice of sequence can significantly impact the expression of an encoded protein. Given the importance of protein-coding sequence and promising industrial and medicinal applications of Clostridium histolyticum collagenase, this study examined the codon optimization of the Col H gene so as to enhance collagenase expression in Escherichia coli (E. coli). Methods: This is an experimental study in which the CDS of Col H gene was optimized according to the codon usage of E. coli, using Gene Designer software (DNA 2.0). Results: The results revealed that relative frequency of codon usage in Col H gene was adapted to the most preferred triplets in E. coli in such a way that codon usage bias in E. coli was enhanced after codon optimization. The higher level of collagenase expression was more likely the result of substituting rare codons with optimal codons. Conclusion: The findings of this study suggest that codon optimization provides a theoretical improvement in Col H gene expression in E. coli. Nevertheless, experimental research is needed to confirm the improvement.
کلیدواژه‌های انگلیسی مقاله Codon, Clostridium histolyticum, Recombinant Proteins, Escherichia coli
نویسندگان مقاله Seyed Mohammad Amin Mahdian |
Department of Pharmaceutical nanotechnology, Faculty of pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Abbasali Raz |
Malaria and Vector Research Group [MVRG], Biotechnology Research Center [BRC], Pasteur Institute of Iran, Tehran, Iran

Navid Dinparast Djadid |
Malaria and Vector Research Group [MVRG], Biotechnology Research Center [BRC], Pasteur Institute of Iran, Tehran, Iran

Hamzeh Alipour |
Research Center for Health Sciences, Institute of Health, Department of Biology and Control of Disease Vectors, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran

نشانی اینترنتی https://jhsss.sums.ac.ir/article_48124_557f6dddba653eb0a84d9361df8ecc38.pdf
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