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Journal of Medical Microbiology and Infectious Diseases، جلد ۹، شماره ۳، صفحات ۱۴۲-۱۴۷
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عنوان انگلیسی Detection of blaOXA-10 and blaOXA-48 Genes in Pseudomonas aeruginosa Clinical Isolates by Multiplex PCR
چکیده انگلیسی مقاله Introduction: The rapidly increasing extended-spectrum β-lactamase-producing Pseudomonas aeruginosa is a threat to health. This study aims to detect the rpoD gene and blaOXA-10 and blaOXA-48 genes in imipenem-resistant P. aeruginosa clinical isolates simultaneously by multiplex polymerase chain reaction. Methods: Eighty-five culture plates were collected from patients suspected of Pseudomonas spp infection in Ghaem Hospital and Shahid Shourideh Clinic in Mashhad from January to February 2021. After biochemical identification of P. aeruginosa isolates and the measurement of antibiotic resistance, blaOXA-10, blaOXA-48, and rpoD genes were investigated by multiplex polymerase chain reaction in the imipenem-resistant isolates. Results: Of 82 P. aeruginosa isolates, 38 (46.34%) were resistant to imipenem, with the highest percentage to carbenicillin (69.5%). All imipenem-resistant P. aeruginosa isolates were confirmed by multiplex PCR using the primers that targeted the rpoD gene. Also, in multiplex PCR, among imipenem-resistant isolates, 10 (26.3%) and 9 (23.6%) had blaOXA-10 and blaOXA-48 genes, respectively. Conclusion: In addition to molecular identification of P. aeruginosa, the present study simultaneously detected blaOXA-10 and blaOXA-48 genes by multiplex PCR. Application of this multiplex PCR, rapid identification of patients, and timely treatment can reduce the β-lactamase gene prevalence in P. aeruginosa clinical isolates.
کلیدواژه‌های انگلیسی مقاله Pseudomonas aeruginosa, Multiplex PCR, rpoD gene, blaOXA-10 gene, blaOXA-48 gene
نویسندگان مقاله | Aghdas Bibi Hashemi
Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran


| Mahboobeh Nakhaei Moghaddam
Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran


| Mohammad Mahdi Forghanifard
Department of Biology, Faculty of Science, Damghan Branch, Islamic Azad University, Damghan, Iran


| Ehsan Yousefi
Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran
نشانی اینترنتی http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-333-1&slc_lang=en&sid=1
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زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Anti-microbial agents, resistance and treatment protocols
نوع مقاله منتشر شده Original article
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