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Journal of Medical Microbiology and Infectious Diseases، جلد ۹، شماره ۲، صفحات ۸۲-۸۷
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عنوان انگلیسی Comparison of PCR with Serology for Detecting Acute Hepatitis A Virus Infection
چکیده انگلیسی مقاله Introduction: Early detection of acute Hepatitis A virus infection (HAV) allows adopting proper treatment measures, rapid recovery, and avoiding side effects. This study compares PCR assay with serology for diagnosing acute HAV infection. Methods: Twenty samples from patients presenting clinical symptoms of acute hepatitis were tested for anti-HAV IgM antibodies. Genomic RNA was extracted from IgM-positive samples, cDNA was synthesized and examined for genomic HAV using a specific HAV real-time detection kit and a nested PCR. Results: Among 20 sera, 14 were positive for anti-HAV IgM antibodies. The specific real-time PCR and nested PCR showed agreement, and both detected HAV genetic material in 3 out of 14 samples. Conclusion: High levels of anti-HAV IgM antibodies do not necessarily indicate acute HAV infection in people presenting clinical symptoms of the disease.  Measuring IgM antibody levels alongside molecular detection of virus genome by DNA-based methods assay can lead to an accurate, timely, and reliable diagnosis of active HAV infection.
کلیدواژه‌های انگلیسی مقاله Molecular diagnostic testing, Serological tests, Hepatitis A viruses
نویسندگان مقاله | Hossein Heydari
Department of biology, North Tehran Branch, Islamic Azad University, Tehran, Iran


| Ahmad Majd
Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran


| Mojtaba Hamidi-Fard
Hepatitis and AIDS Department, Pasteur Institute of Iran, Tehran, Iran & Viral Vaccine Research Center, Pasteur Institute of Iran, Tehran, Iran


| Golnaz Bahramali
Hepatitis and AIDS Department, Pasteur Institute of Iran, Tehran, Iran & Viral Vaccine Research Center, Pasteur Institute of Iran, Tehran, Iran


| Mohammad Reza Aghasadeghi
Hepatitis and AIDS Department, Pasteur Institute of Iran, Tehran, Iran & Viral Vaccine Research Center, Pasteur Institute of Iran, Tehran, Iran
نشانی اینترنتی http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-317-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Diagnostic/screening methods and protocols
نوع مقاله منتشر شده Original article
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