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Journal of Medical Microbiology and Infectious Diseases، جلد ۸، شماره ۳، صفحات ۹۸-۱۰۳
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عنوان انگلیسی Diagnosis of Leptospirosis in Febrile Patients of Golestan Province, Iran: Serology versus PCR
چکیده انگلیسی مقاله Introduction: Leptospirosis is a significant public health problem in the Caspian littoral of Iran comprising Gilan, Mazandaran, and Golestan provinces.  In Golestan province, serology assays indicated anti-Leptospira antibodies in animals and humans; however, no reliable record of infections in patients with signs and symptoms of the disease is available. Methods: We employed the indirect immunofluorescent antibody assay (IFA), and two PCR assays, a real-time PCR (qPCR) and a nested-PCR targeting 16S rDNA (rrs) sequence for diagnosis of leptospirosis in febrile patients in Golestan province. Results: Out of 52 febrile patients, 25 (48.07%) had antibody titers ≥1/80 by IFA, and were defined as positive. In 7, 9, and 7 individuals, the antibody titers were 1/40, 1/20, and 1/10, respectively, and 4 had no antibodies. The qPCR and nested PCR detected leptospiral DNA in 55.75% and 67.3% of the patients' sera, respectively. The two PCR assays had a Kappa agreement of 0.53 (P< 0.0001), suggesting a moderate agreement, and showed a significant reverse association with the IFA titers (P< 0.05). Conclusion: Our results suggest that higher antibody titers accompanied the spirochete's removal from blood by the patient's immune response. Hence, a reliable diagnosis of Leptospira infection necessitates deploying a DNA-based method alongside serology.
کلیدواژه‌های انگلیسی مقاله Leptospirosis, IFA, PCR, Golestan, Iran, Diagnosis, Kappa statistics
نویسندگان مقاله | Saied Reza Naddaf
Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran


| Reyhaneh Mohabati
Department of Virology, Pasteur Institute of Iran, Tehran, Iran


| Rouhollah Vahabpor
Department of Medical Lab Technology, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Sabah Naeimi
Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran


| Sana Eybpoosh
Department of Epidemiology and Biostatistics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran
نشانی اینترنتی http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-26-2&slc_lang=en&sid=1
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زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Diagnostic/screening methods and protocols
نوع مقاله منتشر شده Original article
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