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Journal of Medical Microbiology and Infectious Diseases، جلد ۸، شماره ۲، صفحات ۶۰-۶۴
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عنوان انگلیسی Immunogenicity of the Recombinant Cryptococcus neoformans HSP70, a Potential Candidate for Developing an ELISA Kit
چکیده انگلیسی مقاله Introduction: Cryptococcus neoformans is an encapsulated fungal pathogen that causes life-threatening meningoencephalitis in immunocompromised patients. This yeast secretes several potent immunogenic proteins by secretory vesicular mechanisms, such as HSP70 chaperone. Methods: The PCR-amplified HSP70 gene was cloned into a PET-28a(+) expression vector. The purified recombinant HSP70  (rHSP70) was evaluated by western blotting using an anti-His Tag-HRP antibody and then used for immunization of a rabbit. The serum of the immunized rabbit was tested against the whole lysate of C. neoformans in ELISA. Results: The antibodies in the rabbit's serum recognized lysate of C. neoformans yeast. The highest antibody levels were achieved after the third booster injection. Conclusion: The rHSP70 showed to be a reliable candidate for the designing and development of an ELISA kit for early detection of cryptococcosis, and to screen a large number of specimens.
کلیدواژه‌های انگلیسی مقاله Cryptococcus neoformans, Enzyme-Linked Immunosorbent Assay, Heat Shock Protein 70KD
نویسندگان مقاله | Shima Safaei
Department of Pathobiology, School of Paraveterinary Sciences, BU-Ali Sina University, Hamadan, Iran.


| Hossein Rezvan
Department of Pathobiology, School of Paraveterinary Sciences, BU-Ali Sina University, Hamadan, Iran.


| Roohollah Fateh
Department of Microbiology and Immunology, School of Medicine, Qom University of Medical Sciences, Qom, Iran.


| Mohammad Khalifeh Gholi
Department of Microbiology and Immunology, School of Medicine, Qom University of Medical Sciences, Qom, Iran.
نشانی اینترنتی http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-234-2&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Diagnostic/screening methods and protocols
نوع مقاله منتشر شده Original article
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