این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Caspian Journal of Enviromental Sciences، جلد ۱۹، شماره ۴، صفحات ۶۲۹-۶۳۷
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی The change in heat inactivation of Escherichia coli O157:H7 after entering into the viable but non-culturable state in salted fish, Hypophthalmichthys molitrix
چکیده انگلیسی مقاله Many species of non-sporulating bacteria including Escherichia coli can enter into a viable but non-culturable (VBNC) state under stress conditions. In this study, the change in thermal resistance of E. coli O157:H7 after entering into the VBNC state in salted silver carp, Hypophthalmichthys molitrix, was investigated. E. coli O157:H7 was inoculated on the fish control group (TF) and on those fish with 30% NaCl (TF + 30% NaCl) at room temperature. Culturability of bacteria was determined using routine culture and colony counting on Sorbitol MacConkey agar. When bacteria were non-cultivable, the RT-PCR of 16S rRNA gene (including direct extraction and purification of RNA, DNase I treatment for removing DNA contamination, cDNA synthesis and electrophoresis of PCR products of cDNA) was used to detect VBNC E. coli O157:H7. Also, cultivable and VBNC E. coli O157:H7 were individually heat-treated at 55, 62 and 70 °C for 5 min. The samples were cooled and after 24 h, the thermal resistance of bacteria was determined through viability detection using RT-PCR of 16S rRNA gene. The culturability of bacteria was kept in fish treatment but they were non-cultivable in fish under 30% NaCl after 5 days. The positive expression of 16S rRNA in all studied treatments indicated the entering of E. coli O157:H7 into the VBNC state in fish treatment under 30% NaCl. Moreover, the RT-PCR of 16S rRNA gene showed that only VBNC forms of E. coli O157:H7 showed viability at 62 °C for 5 min which indicated the increased resistant of VBNC bacteria to the thermal inactivation. The ability of E. coli O157:H7 to enter into VBNC state in salted fish and also the increase of its thermal resistance suggest that VBNC E. coli O157: H7 can be considered as critical threat to public health and food safety.
کلیدواژه‌های انگلیسی مقاله Escherichia coli O157 H7, VBNC state, Thermal resistance
نویسندگان مقاله Mohammad Khezri |
Department of Seafood Science, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran

Masoud Rezaei |
Department of Seafood Science, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran

Ashraf Mohabbati Mobarez |
Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Mehdi Zolfaghari |
Fisheries Department, Agriculture and Natural Resource University of Gorgan, Gorgan, Iran

نشانی اینترنتی https://cjes.guilan.ac.ir/article_5136_34350fb37be0093b2609c2b09c64f231.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات