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Iranian Journal of Fisheries Sciences، جلد ۲۰، شماره ۵، صفحات ۱۳۱۷-۱۳۳۵
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عنوان انگلیسی Research Article: Detection of betanodavirus in wild golden grey mullet (Chelon aurata) in southern parts of the Caspian Sea using Real-time RT-PCR and immunohistochemistry
چکیده انگلیسی مقاله Viral Nervous Necrosis (VNN), caused by betabodavirus, is considered as one of the most important threats for mariculture and aquaculture globally. The disease was previously reported in wild golden grey mullet (Chelon aurata) from the Caspian Sea in 2004. To update these findings and to examine the phylogenetic relationship of the betanodavirus involved in disease outbreaks in Iran with other betanodavirus genotypes, forty golden grey mullets were screened for the presence of betanodavirus using real-time RT-PCR targeting the T4 region of RNA2 coat protein gene. The results showed that all 40 fish has positive test for the virus, which was also confirmed by immunohistochemistry. The phylogenetic relationship of betanodavirus involved in disease outbreaks in Iran from 2016 to 2017 was compared with betanodavirus genotypes sequenced from elsewhere, and the 300 bp product from the real-time RT-PCR sequenced was found to have a 98–100% homology with red-spotted grouper nervous necrosis virus (RGNVV), confirming that the betanodavirus detected in wild golden grey mullet in the southern parts of the Caspian Sea adjacent to Iran determined to be the RGNNV genotype.
کلیدواژه‌های انگلیسی مقاله Betanodavirus, Viral Nervous Necrosis, Phylogenetic analysis, Real-time RT-PCR, Immunohistochemistry, Golden grey mullet.
نویسندگان مقاله | F. Hassantabar
1Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, PO Box: 578, Sari, I.R. Iran.


| M.J. Zorriehzahra
Scientific Information and Communication Dept., Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Tehran, I.R. Iran.


| F. Firouzbakhsh
Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, PO Box: 578, Sari, I.R. Iran


| K.D. Thompson
3Aquaculture Research Group, Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian, EH26 0PZ, UK
نشانی اینترنتی http://jifro.ir/browse.php?a_code=A-10-1784-2&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده fish disease
نوع مقاله منتشر شده پژوهشی
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