این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Molecular Biology Research Communications، جلد ۱۰، شماره ۲، صفحات ۷۳-۸۳
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Abundance of antibiotic resistance genes in bacteria and bacteriophages isolated from wastewater in Shiraz
چکیده انگلیسی مقاله Generally, the high widespread presence of antimicrobial resistance, and the next freeing into aquatic environments which provide a situation for transmission of these genes in water is because of the abuse of the antimicrobial drugs in both medicine and veterinary medicine. In aquatic environment, bacteriophages could have an important role in sharing antimicrobial resistance genes. The purpose of this study was to assess three important antibiotic resistance genes including two β-lactamases (blaTEM, blaSHV) and sul1 gene, referring to resistance to sulfonamides, in both bacteria and phage DNA fractions of wastewater samples, Shiraz, Iran, using polymerase chain reaction. The prevalence of those genes was extremely high and equal to 100% in bacterial DNA, while these rates were lower in phage DNA fractions as 66.66%, 66.66% and 58.33% for blaTEM, blaSHV and sul1, respectively. In conclusion, detection of mentioned genes in bacterial and phage DNA fractions from ambient water is considerable, so the possibility of harbouring and transferring of antibiotic resistance genes by phages needs to be explored in the future. Also, available data is a reputable endorsement that wastewater is a hotspot for these kinds of genes to spread in the environment. Based on our knowledge, this is the first report of blaTEM and bla SHV and sul1 genes in bacterial and phage DNA fractions insulated from urban wastewater and environment in Iran.
کلیدواژه‌های انگلیسی مقاله antibiotic resistance gene, Bacteria, Phage, β-lactamases, Sulfonamides
نویسندگان مقاله Sahar Zare |
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Abdollah Derakhshandeh |
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Ali Mohammadi |
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Masoud Noshadi |
Department of Water Science and Engineering, School of Agriculture, Shiraz University, Shiraz, Iran

نشانی اینترنتی https://mbrc.shirazu.ac.ir/article_6081_ddcf0413e2565a83c7c1bd212a226f32.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات