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Journal of Reproduction and Infertility، جلد ۲۲، شماره ۴، صفحات ۲۵۱-۲۵۸
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عنوان انگلیسی Clinical Outcomes of Rescue Intracytoplasmic Sperm Injection at Different Timings Following In Vitro Fertilization
چکیده انگلیسی مقاله Background: Although rescue intracytoplasmic sperm injection (r-ICSI) is extensively used worldwide, the indication of r-ICSI and its optimal timing remains obscure. This study aimed to assess the outcomes of r-ICSI following in vitro fertilization in different timings when fertilization is confirmed. Methods: This study included 5,156 cycles (47,785 eggs). Fertilization was confirmed by polar body analysis after 4 and 6 hr of coincubation of the sperm and oocyte. Oocytes that underwent IVF were divided into two groups based on the time when a second polar body was detected in more than 30% of all oocytes (Four-hr group and six-hr group). If the second polar body was not detected or was present in less than 30% of all oocytes after six hr of coincubation, rescue-ICSI (r-ICSI) was performed for oocytes without a second polar body (r-ICSI group). Results: The fertilization rates of two pronuclear (2PN) oocytes in the three groups (Four-hr group, six-hr group, and r-ICSI group) were 70.7%, 51.3%, and 58.0%, respectively. The blastocyst formation rates were 62.8%, 53.4%, and 42.9%, respectively. Conclusion: Performing r-ICSI after six hr of coincubation can salvage cases with fertilization failure in IVF. The higher fertilization rate of r-ICSI indicates that all oocytes without signs of fertilization after six hr of coincubation should undergo r-ICSI.
کلیدواژه‌های انگلیسی مقاله Assisted reproductive techniques, Fertilization failure, In vitro fertilization, Infertility, Intracytoplasmic sperm injection
نویسندگان مقاله | Yuki Shiraiwa
Hanabusa Women’s Clinic, Hyogo, Japan


| Noritoshi Enatsu
Hanabusa Women’s Clinic, Hyogo, Japan


| Kazuki Yamagami
Hanabusa Women’s Clinic, Hyogo, Japan


| Koyu Furuhashi
Hanabusa Women’s Clinic, Hyogo, Japan


| Toshiroh Iwasaki
Hanabusa Women’s Clinic, Hyogo, Japan


| Junko Otsuki
Okayama University Assisted Reproduction Technology Center, Okayama, Japan


| Masahide Shiotani
Hanabusa Women’s Clinic, Hyogo, Japan
نشانی اینترنتی https://www.jri.ir/article/120137
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