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Iranian Journal of Microbiology، جلد ۱۴، شماره ۲، صفحات ۱۶۱-۱۶۷
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عنوان انگلیسی Detection of bacterial agents causing prostate infection by culture and molecular methods from biopsy specimens
چکیده انگلیسی مقاله Background and Objectives: Prostatitis affects about 16% of men in their lifetime and sometimes leading to prostate cancer. Bacterial infections are the most common causes of prostatitis. Diagnosis of the causative agents of bacterial prostate infections plays an essential role in timely treating and preventing secondary complications. This study isolated bacterial infectious agents in patients’ surgical prostate and evaluated them by routine and molecular microbiological methods. Materials and Methods: In this cross-sectional study, 72 prostate biopsy specimens were collected from the Orology Departmen of hospitals of Qazvin University of Medical Sciences. All samples were cultured in aerobic and anaerobic conditions. Antibiotic susceptibility test by Kirby-Bauer standard method was performed for all isolated bacteria. In addition, all isolated bacteria were identified using 16S rDNA PCR and sanger sequencing methods. Also, TaqMan real-time PCR was applied to detect Ureaplasm aurealyticum, Mycoplasma hominins, and Mycoplasma genitalium. Results: In conventional culture method, out of 18 positive samples, 15 samples (83.3%) were Gram-negative bacteria and 3 samples (16.6%) were Gram-positive bacteria, containing Escherichia coli (55.5%), Klebsiella pneumoniae (11.1%), Enterobacter cloacae (5.5%), Pseudomonas aeruginosa (11.1%), Staphylococcus aureus (11.1%), and Enterococcus faecalis (5.5%). The results of molecular identification methods were the same as conventional culture results. Also, four patients were Ureaplasm aurealyticum, and three patients were positive for Mycoplasma hominis. Conclusion: Most bacteria isolated from prostate specimens belonged to the Enterobacteriaceae family, especially Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae. Staphylococcus aureus and Enterococcus faecalis were cocci isolated in the specimens too. Also, Ureaplasma urealyticum, and Mycoplasma hominis were identified in prostatitis.
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نویسندگان مقاله | Ali Akbar Karami
Department of Urology, Qazvin University of Medical Sciences, Qazvin, Iran


| Amir Javadi
Department of Social Sciences, School of Medicine, Qazvin University of Medical Sciences Qazvin, Iran


| Sohrab Salehi
Department of Urology, Qazvin University of Medical Sciences, Qazvin, Iran


| Neda Nasirian
Department of Pathology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran


| Amirhosein Maali
Department of Immunology, Pasteur Institute of Iran, Tehran, Iran; Department of Medical Biotechnology, Faculty of Allied Medicine, Qazvin University of Medical Sciences, Qazvin, Iran


| Maryam Bakhshalizadeh Shadkam
Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran


| Masoumeh Najari
Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran


| Zahra Rousta
Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran


| Safar Ali Alizadeh
Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/3438
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