این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Middle East Journal of Digestive Diseases، جلد ۱، شماره ۱، صفحات ۱۲۴-۱۳۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Simultaneous Hepatitis C Virus Genotyping and Variant Detection in Patients with Thalassemia: A Single-Center Phylogenetic Study
چکیده انگلیسی مقاله BACKGROUND: Hepatitis C virus (HCV) genotype distribution is different in various regions. A variety of strategies could be used to detect HCV genotypes and subtypes. The aim of the present study was to introduce a genotyping method by an in-house protocol that could be used to determine HCV drug-resistant variants and phylogeny studies. METHODS: Samples from 91 patients with thalassemia were used for HCV genotyping by Cobas 4800 platform, and 50 cases of 1a, 1b, and 3a genotypes underwent amplification and sequencing of NS5A and NS5B by using consensus primers via conventional reverse transcription-polymerase chain reaction (RT-PCR) (please spell out) method. An ABI 3730xl system used for direct sequencing. Raw sequences were analyzed by popular bioinformatics software MEGA6 and CLC workbench 5. Phylogenetic construction was drawn using 1000 replicates bootstrap by the neighbor-joining method. Multiple sequence alignment (MSA) was performed for mutation detection. RESULTS: Sequencing results of 50 HCV isolates subtypes 1a (31/45), 3a (15/22) and 1b (4/8) NS5A and NS5B genes showed there were 72 NS5A and 105 NS5B mutations. Moreover, 8 resistant associated substitutions (RASs) (please spell out) were identified in nine thalassemia cases by multiple sequence alignment (MSA) (please spell out) protein analysis. The phylogenetic tree construct drew confirmed by the Cobas HCV genotyping results. CONCLUSION: The phylogenetic analysis could be a useful tool for HCV genotyping in case of determining the drug-resistant substitutions; however, it is time-consuming and needs expert analysis and interpretation. This preliminary study in Iranian patients with thalassemia introduces specific conventional RT-PCR to find RASs to direct acting antivirals (DAAs) (please spell out) and subtype determination at the same time.
کلیدواژه‌های انگلیسی مقاله Hepatitis C virus (HCV),Genotyping,Phylogeny,Thalassemia
نویسندگان مقاله | Fahimeh Safarnezhad Tameshkel
Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran Department of Virology, Iran University of Medical Sciences, Tehran, Iran


| Mohammad Hadi Karbalaie Niya
Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran


| Farhad Zamani
Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran


| Hossein Ajdarkosh
Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran


| Mahmoodreza Khoonsari
Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran


| Amir Hossein Faraji
Department of Social Medicine, Zanjan University of Medical Sciences, Zanjan, Iran


| Nima Motamed
Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran


| Mehdi Nikkhah
Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran


| Mitra Ameli
Nedmedica, Heerlen, Netherlands


| Seyyed Mohammad Miri
Pediatric Hematology Oncology, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Thalassemia Clinic, Tehran, Iran


| Azita Azarkeivan
Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran


| Masood Reza Sohrabi
Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran


| Hossein Keyvani


نشانی اینترنتی http://mejdd.org/index.php/mejdd/article/view/2740
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi) 10.15171/middle east j di.v1i1.2740
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات