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Iranian Journal of Microbiology، جلد ۱۴، شماره ۳، صفحات ۳۱۹-۳۲۷
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عنوان انگلیسی The molecular characterization of colistin-resistant isolates of Acinetobacter baumannii from patients at intensive care units
چکیده انگلیسی مقاله Background and Objectives: The objective of this study was to determine molecular characterization and genetic diversity of colistin-resistant A. baumannii clinical isolates in Intensive Care Unit hospitalized patients. Materials and Methods: A total of 127 A. baumannii clinical isolates were evaluated for antimicrobial susceptibility. PCR reaction and sequencing were performed for the detection of mutations in pmrAB and lpx ACD genes. Results: Based on antimicrobial susceptibility testing, 40.94% and 33.85% of the isolates were MDR and XDR respectively whereas 3.93% of them were found to be PDR. Results of agar dilution MIC and E-test indicated that 76% of the isolates were sensitive to colistin. All of the isolates were positive for blaOXA-51 and 50% of them were positive for both blaOXA-23-like and blaOXA-143-like genes while only 25% of the isolates were positive for blaOXA-72. None of them were positive for the blaOXA-58-like gene. There is no mutation in pmrA. The V162A substitution for pmrB gene was repeated in two isolates, and E394 D and Y292H substitutions in lpxA were observed in two isolates; also, C120R and F165L substitutions in lpxC gene was repeated in two isolates. Analysis of phylogenetic tree based on alterations in lpxACD and pmrB genes indicated the appearance of new isolates compared to the reference strain ATCC17978 A. baumannii isolates. Conclusion: The present study indicated the prevalence of MDR and XDR A. baumannii isolates and the emergence of PDR isolates in the northwest portion of Iran. The appearance of colistin-resistant isolates with new mutations in pmrB, lpxACD genes indicates new resistance mechanisms.
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نویسندگان مقاله | Safar Farajnia
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran


| Fariba Lotfi
Department of Biological Sciences, Faculty of Basic Sciences, Higher Education Institute of Rab-Rashid, Tabriz, Iran


| Alireza Dehnad
Department of Biotechnology, East Azerbaijan Research and Education Center Agricultural and Natural Resources, Razi Vaccine and Serum Research Institute, AREEO, Tabriz, Iran


| Maryam Shojaie
Department of Biological Sciences, Faculty of Basic Sciences, Higher Education Institute of Rab-Rashid, Tabriz, Iran


| Roya Raisi
Department of Biological Sciences, Faculty of Basic Sciences, Higher Education Institute of Rab-Rashid, Tabriz, Iran


| Leila Rahbarnia
Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran


| Ahad Bazmani
Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran


| Behrooz Naghili
Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran


| Samaneh Shiry
Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/3400
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