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Middle East Journal of Digestive Diseases، جلد ۱۴، شماره ۲، صفحات ۱۹۲-۱۹۹
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عنوان انگلیسی Introducing New Potential Biomarkers for Celiac Disease among the Genes Extracted from General Databases
چکیده انگلیسی مقاله BACKGROUND: Inflammatory cytokines play roles in the pathogenesis of celiac disease. To introduce new diagnostic markers in patients with celiac disease for easy, fast, low cost, and non-invasive diagnosis, we evaluated the peripheral blood expression levels of Interleukin 15 (IL15), Interleukin 17A (IL17A), Interleukin23A (IL23A), granzyme B (GZMB), T-Box Transcription Factor 21 (TBX21), and TNF Alpha Induced Protein 3 (TNFAIP3) of patients compared with the healthy controls, which were extracted from public databases organized in a protein-protein interaction network, in this group. METHODS: Peripheral blood mononuclear cells were collected from 30 patients with celiac disease and 30 healthy subjects. Total RNA was extracted, and mRNA expression levels of targeted genes were investigated by the quantitative real-time Polymerase chain reaction (PCR) method. SPSS software was used for statistical analysis. Receiver operating characteristic curve (ROC curve) analysis was performed to characterize the diagnostic ability of the studied genes. RESULTS: The expression of IL15, IL17A, IL23A, GZMB, TBX21, and TNFAIP3 genes in peripheral blood mononuclear cells of patients with celiac disease showed a significant increase compared with the control group. Among them, TNFAIP3, IL23A, and GZMB have better resolution and diagnostic value in differentiating patients with celiac disease from healthy controls. CONCLUSIONS: Our results suggest that TNFAIP3, IL23A, and GZMB could be useful and sensible markers in differentiating patients with celiac disease from healthy controls. However, the diagnostic relevance of other genes recognized by pathway analysis needs to be further investigated.
کلیدواژه‌های انگلیسی مقاله Autoimmune diseases,Blood,Celiac disease,Inflammation,Polymerase chain reaction
نویسندگان مقاله | Ensieh Khalkhal
Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Mostafa Rezaei-Tavirani
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Nastaran Asri
Chemical Injuries Research Center, Systems Biology and Poisoning Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran


| B. Fatemeh Nobakht M. Gh
Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Somayeh Jahani-Sherafat
Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Mohammad Hosain Haidari
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Mohammad Rostami-Nejad


نشانی اینترنتی http://mejdd.org/index.php/mejdd/article/view/2615
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