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Basic and Clinical Neuroscience، جلد ۱۳، شماره ۵، صفحات ۰-۰
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چکیده فارسی مقاله
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عنوان انگلیسی Transdifferentiation of Human Umbilical Cord- Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture
چکیده انگلیسی مقاله Introduction: The induction of from human umbilical cord-derived mesenchymal stem cells (HUC-MSCs) toward dopaminergic neurons is a major challenge in   tissue engineering and experimental and clinical treatments of various neurodegenerative diseases including Parkinson's disease. This study aimed to differentiate HUC-MSCs into dopaminergic neuron-like cells. Methods: After HUC-MSCs isolation and characterizations, they transferred onto matrigel- coated plates and incubated with a  cocktail of dopaminergic neuronal differentiation factors. The capacity of  differentiation into dopaminergic neuron-like cells in 2D culture and on matrigel was assessed by real-time PCR, immunocytochemistry and high-performance liquid chromatography (HPLC). Results: Our results showed that the transcript and protein levels of dopaminergic neuronal markers  was significantly increased on  Matrigel differentiated cells as compared with  2D culture plates. Conclusions: Overall, the results of this study suggest that HUC-MSCs can successfully differentiate toward dopaminergic neuron-like cells on Matrigel, having great potentials for the treatment of dopaminergic neuron-related diseases.
کلیدواژه‌های انگلیسی مقاله Differentiation, Dopaminergic Neurons, Human Umbilical Cord Mesenchymal Stem Cells, In Vitro
نویسندگان مقاله | Ardeshir Moayeri
Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran


| Rafieh Alizadeh
ENT and Head & Neck Research Center and Department, The five senses institute, Iran University of Medical Sciences, Tehran, Iran.


| Hatef Ghasemi Hamidabadi
Department of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.


| Maryam Nazm Bojnordi
Department of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran


| Ali Niapour
Research Laboratory for Embryology and Stem Cells, Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.


| Azim Hedayatpour
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.


| Marzieh Darvishi
Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.


| Fatemeh Heidari
Anatomy Department, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran.


| Maryam Soleimani
Department of Basic Sciences, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.


| Leila Elyasi
Neuroscience Research Center, Department of Anatomy, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
نشانی اینترنتی http://bcn.iums.ac.ir/browse.php?a_code=A-10-973-3&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Cellular and molecular Neuroscience
نوع مقاله منتشر شده Original
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