این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Basic and Clinical Neuroscience، جلد ۱۳، شماره ۵، صفحات ۰-۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Human Olfactory Ectomesenchymal Stem Cells (OE-MSCs) Display Schwann Cell-Like Phenotypes and Promote Neurite Outgrowth In Vitro
چکیده انگلیسی مقاله Strategies of Schwann cell (SC) transplantation for regeneration of peripheral nerve injury involve many limitations. Stem cells can be used as alternative cell source for differentiation into Schwann cells. Given the high potential of neural crest-derived stem cells for the generation of multiple cell lineages, in this research, we considered whether olfactory ectomesenchymal stem cells (OE-MSCs) derived from neural crest can spontaneously differentiate into SC lineage. OE-MSCs were isolated from human nasal mucosa and characterized by the mesenchymal and neural crest markers. The cells were cultured in glial growth factors-free medium and further investigated in terms of the phenotypic and functional properties. Immunocytochemical staining and real-time PCR analysis indicated that the cultured OE-MSCs expressed SCs markers, SOX10, p75, S100, GFAP and MBP, differentiation indicative. It was found that the cells could secrete neurotrophic factors, including brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). Furthermore, after co-cultured with PC12, the mean neurite length was enhanced by OE-MSCs. The findings indicated that OE-MSCs could be differentiated spontaneously into SC-like phenotypes, suggesting their applications for transplantation in peripheral nerve injuries.  
کلیدواژه‌های انگلیسی مقاله Spontaneous differentiation, Olfactory ectomesenchymal stem cells, Schwann cell, Peripheral nerve regeneration
نویسندگان مقاله | Maedeh Entezari
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.


| Mehrdad Bakhtiari
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.


| Fatemeh Moradi
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.


| Masoud Mozafari
Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.


| Zohreh Bagher
ENT and Head & Neck Research Center and Department, The Five Senses Institute, Hazrat Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.


| Mansoureh Soleimani
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
نشانی اینترنتی http://bcn.iums.ac.ir/browse.php?a_code=A-10-3542-1&slc_lang=en&sid=1
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Cellular and molecular Neuroscience
نوع مقاله منتشر شده Original
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات