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Iranian Journal of Toxicology، جلد ۱۶، شماره ۴، صفحات ۰-۰
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عنوان انگلیسی Nickel Increases Chromosomal Abnormalities by Interfering with the Initiation of DNA Repair Pathways
چکیده انگلیسی مقاله Background: Nickel is a carcinogenic, heavy metal released through industrial activities and via natural resources. It is able to cause DNA damages by reducing the efficiency of DNA repair mechanisms. However, the exact time point at which it is able to interfere with these mechanisms is not yet clearly understood. Methods: To find the most nickel-vulnerable time of repair mechanisms, human dermal fibroblasts (HDF) were treated with three doses of nickel before and after X-irradiation. The induced frequency of chromosomal abnormality was studied using micronucleus assay in binucleated cells. The cytotoxicity of different treatments was established using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: The results revealed that nickel treatment had a synergistic effect on inducing Micronucleus frequency only when cells were treated 2hr before X-irradiation. The X-ray treatment of the cells with 5- and 10-mM nickel had a cytotoxic effect mainly when given 6hr after the irradiation. Conclusions: The results suggest that nickel can interfere with human DNA repair mechanisms only at the start of the process, while having no significant effect on the human DNA repair mechanisms when activated.  
کلیدواژه‌های انگلیسی مقاله Chromosomes, DNA repair mechanisms, Human dermal fibroblast (HDF), Micronucleus assay, Nickel, X-irradiation
نویسندگان مقاله | Mahsa Ghorbani
Genetics, Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.


| Farhang Haddad
Cytogenetics, Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.


| Khadije Shahrokhabadi
Molecular Genetics, Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
نشانی اینترنتی http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-439-2&slc_lang=en&sid=1
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