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Iranian Journal of Public Health، جلد ۵۱، شماره ۱۱، صفحات ۲۴۹۴-۲۵۰۳
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عنوان انگلیسی Effect of miRNA-218-5p on Proliferation, Migration, Apoptosis and Inflammation of Vascular Smooth Muscle Cells in Abdominal Aortic Aneurysm and Extracellular Matrix Protein
چکیده انگلیسی مقاله Background: To explore the effects of miRNA-218-5p on inflammation and extracellular matrix proteins of vascular smooth muscle cell line in abdominal aortic aneurysm (AAA). Methods: miR-218-5p expression was detected with RT-qPCR. The proliferative activity of vascular smooth muscle cells (VSMCs) was detected with CCK-8, the migration was detected by Transwell, and the apoptosis was detected with flow cytometry. The expression levels of inflammatory factors (IL-1β and IL-18) were detected by ELISA. The expression levels of proteins (MMP-9 and Netrin-1) and ADAMTS5 were detected by Western blot. The targeting relationship between miR-218-5p and ADAMTS5 was verified with dual-luciferase reporter assay. Results: Up-regulating miR-218-5p could significantly inhibit the proliferation and migration of VSMCs and induced the apoptosis (P< 0.05). Down-regulating miR-218-5p could significantly promote the proliferation and migration of VSMCs and inhibit the apoptosis (P< 0.05). Up-regulating miR-218-5p could inhibit the expression levels of THP-1 cytoinflammatory factors (IL-8 and IL-1β), MMP-9 and netrin-1. ADAMTS5 was the target gene of miR-218-5p. When there were both overexpression of ADAMTS5 and upregulation of miR-218-5p, the upregulation of miR-218-5p could alleviate the effects of overexpression of ADAMTS5 on the proliferation, migration and apoptosis of VSMCs. Conclusion: miR-218-5p/ADAMTS-5 molecular axis regulates the proliferation, migration, and apoptosis of VSMCs, as well as the expression of THP-1 cell inflammatory molecules and extracellular matrix molecules.
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نویسندگان مقاله | Min Hu
Department of Vascular Surgery, Wuhan Central Hospital, Tongji Medical College, Huazhong University of Science and Tech-nology, Wuhan 430000, China


| Hui Qiu
Department of Oncology, Wuhan NO.1 Hospital, Wuhan 430000, China


| Tao He
Department of Vascular Surgery, Wuhan Central Hospital, Tongji Medical College, Huazhong University of Science and Tech-nology, Wuhan 430000, China


| Minyu Zhong
Department of Oncology, Wuhan NO.1 Hospital, Wuhan 430000, China
نشانی اینترنتی https://ijph.tums.ac.ir/index.php/ijph/article/view/28212
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