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Iranian Journal of Microbiology، جلد ۱۴، شماره ۶، صفحات ۸۵۰-۸۶۲
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عنوان انگلیسی The inhibitory effect of dextranases from Bacillus velezensis and Pseudomonas stutzeri on Streptococcus mutans biofilm
چکیده انگلیسی مقاله Background and Objectives: Dental caries is a breakdown of the teeth enamel due to harmful bacteria, lack of oral hygiene, and sugar consumption. The acid-producing bacterium Streptococcus mutans is the leading cause of dental caries. Dextranase is an enzyme that can degrade dextran to low molecular weight fractions, which have many therapeutic and industrial applications. The purpose of the present study was to isolate a novel dextranase-producing bacteria from a source (molasses). The cell-free extracts containing dextranases were tested as antibiofilm agents. Materials and Methods: Dextranase-producing bacteria were identified using phenotypic and genotypic methods such as 16S rRNA gene sequencing and enzymatic characterization. Results: The highest six dextranase-producing bacterial isolates were Bacillus species. The best conditions for dextranase productivity were obtained after 72 hours of culture time at pH 7. The addition of glucose to the medium enhanced the production of the enzymes. The cell-free extract of the six most active isolates showed remarkable activity against biofilm formation by Streptococcus mutans ATCC 25175. The highest inhibition activities reached 60% and 80% for Bacillus velezensis and Pseudomonas stutzeri, respectively. Conclusion: Therefore, our study added to the current dextranase-producing bacteria with potential as a source of dextranases.
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نویسندگان مقاله | Samah Mahmoud
Department of Microbiology and Immunology, Faculty of Pharmacy, NAHDA-University, Beni-Suef, Egypt; Department of Microbiology and Immunology, Faculty of Medicine, Merit University, New Sohag, Egypt


| Yasser Gaber
Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt; Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Mutah University, Al-Karak, Jordan


| Rania Khattab
Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt


| Walid Bakeer
Department of Microbiology and Immunology, Faculty of Pharmacy, NAHDA-University, Beni-Suef, Egypt; Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt


| Tarek Dishisha
Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt


| Mohamed Ramadan
Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/3857
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