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Iranian Journal of Basic Medical Sciences، جلد ۲۶، شماره ۳، صفحات ۳۵۱-۳۵۸
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عنوان انگلیسی Protective effects of baicalin against L-glutamate-induced oxidative damage in HT-22 cells by inhibiting NLRP3 inflammasome activation via Nrf2/HO-1 signaling
چکیده انگلیسی مقاله Objective(s): To explore the ability and underlying molecular mechanisms involved in the protective effects of Baicalin (BA) against L-Glutamate-induced mouse hippocampal neuron cell line HT-22.Materials and Methods: The cell injury model of HT-22 cells was induced by L-glutamate, and cell viability and damage were detected by CCK-8 and LDH assays. Generation of intracellular reactive oxygen species (ROS) was measured by DCFH-DA in situ fluorescence method. The SOD activity and MDA concentration in the supernatants were determined by WST-8 and colorimetric method, respectively. Furthermore, Western blot and real-time qPCR analysis were utilized to detect the expression levels of the Nrf2/HO-1 signaling pathway and NLRP3 inflammasome proteins and genes.Results: L-Glutamate exposure induced cell injuries in HT-22 cells, and the concentration of 5 mM L-Glutamate was chosen to be the modeling condition. Co-treatment with BA significantly promoted cell viability and reduced LDH release in a dose-dependent manner. In addition, BA attenuated the L-Glutamate-induced injuries by decreasing the ROS production and MDA concentration, while increasing the SOD activity. Moreover, we also found that BA treatment up-regulated the gene and protein expression of Nrf2 and HO-1, and then inhibited the expression of NLRP3.Conclusion: Our study found that BA could relieve oxidative stress damage of HT-22 cells induced by L-Glutamate, and the mechanism might be related to the activation of Nrf2/HO-1 and inhibition of NLRP3 inflammasome.
کلیدواژه‌های انگلیسی مقاله Baicalin, L-Glutamate, NLRP3 inflammasome, Nrf2/HO-1 signaling - pathway, Oxidative stress
نویسندگان مقاله | Junyuan Li
Institute of Basic Medical Sciences, Xiyuan Hospital of China Academy of Chinese Medical Sciences, No.1 Xiyuan Caochang, Haidian District, Beijing, 100091, China|Key Laboratory of Pharmacology of Chinese Materia Medica, Beijing 100091, China


| Gang Wang
Hubei Provincial Hospital of Integrated Chinese and Western Medicine, Wuhan, Hubei 430015, China


| Yehao Zhang
Institute of Basic Medical Sciences, Xiyuan Hospital of China Academy of Chinese Medical Sciences, No.1 Xiyuan Caochang, Haidian District, Beijing, 100091, China|Key Laboratory of Pharmacology of Chinese Materia Medica, Beijing 100091, China


| Xiaodi Fan
Institute of Basic Medical Sciences, Xiyuan Hospital of China Academy of Chinese Medical Sciences, No.1 Xiyuan Caochang, Haidian District, Beijing, 100091, China|Key Laboratory of Pharmacology of Chinese Materia Medica, Beijing 100091, China


| Mingjiang Yao
Institute of Basic Medical Sciences, Xiyuan Hospital of China Academy of Chinese Medical Sciences, No.1 Xiyuan Caochang, Haidian District, Beijing, 100091, China|Key Laboratory of Pharmacology of Chinese Materia Medica, Beijing 100091, China
نشانی اینترنتی https://ijbms.mums.ac.ir/article_21727.html
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