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Iranian Journal of Basic Medical Sciences، جلد ۲۵، شماره ۱۰، صفحات ۱۲۳۴-۱۲۴۲
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عنوان انگلیسی Conjugated PNC-27 peptide/PEI-Superparamagnetic iron oxide nanoparticles (SPIONs) as a double targeting agent for early cancer diagnosis: In vitro study
چکیده انگلیسی مقاله Objective(s): Superparamagnetic iron oxide nanoparticles (SPIONs) have been considered promising non-invasive imaging tools in medicine. However, their high surface energy leads to NPs aggregation, while non-targeted SPIONs can cause cytotoxic effects on normal cells. In this work, we evaluated the in vitro potential of polyethyleneimine (PEI)-SPIONs targeted by PNC27 peptide as a double targeting agent throughout early cancer diagnosis.Materials and Methods: Initially, PEI was conjugated to PNC27 with HDM-2-binding domain. Then, SPIONs were loaded into PEI-PNC27 through the ligand exchange method. The physicochemical characteristics of the synthesized NPs were evaluated. The cytotoxicity and targeting efficiency were assayed against HT-29 and CT-26 cell lines along with NIH-3t3 as normal cells by MTT method and Prussian blue staining test, respectively. Results: The mean diameter of synthesized carriers was obtained in the range of 86.6 – 116.1 nm with a positive charge. According to the cytotoxicity results, the binding and uptake abilities of the PNC27 peptide by cancer cells were significantly higher than that of the NIH-3t3 cells. However, the results were indicative of the more toxic impacts of targeted synthesized NPs against CT-26 cancer cell line when being compared with HT-29 cells, which may be caused by the different cytotoxicity mechanisms of NPs. In addition, the targeted carriers and SPIONs were present inside and around the cells with HDM-2 expression along with only a few non-targeted vectors, while displaying no appearance throughout the normal cell.Conclusion: The results indicated the efficiency of targeted PEI-coated SPIONs for cancer diagnostic applications.
کلیدواژه‌های انگلیسی مقاله B-PEI, Cytotoxicity effect, Iron oxide, PNC27 peptide, SPIONs, Targeted cancer diagnostic
نویسندگان مقاله | Reihaneh Rahmani
Department of Laboratory Sciences, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran|Research Institute of Applied Sciences (ACECR), Shahid Beheshti University, Tehran, Iran


| Majid Darroudi
Nuclear Medicine Research Center, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Medical Biotechnology and Nanotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


| Mohsen Gharanfoli
Research Institute of Applied Sciences (ACECR), Shahid Beheshti University, Tehran, Iran|Department of Cell and Molecular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran


| Jamshidkhan Chamani
Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran


| Mehran Gholamin
Department of Laboratory Sciences, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran|Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran


| Maryam Hashemi
Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran|Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
نشانی اینترنتی https://ijbms.mums.ac.ir/article_21073.html
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